文献类型： 外文期刊

作者： Zhao, Mingxiu ¹ ; Yang, Yi ¹ ; Shi, Yue ¹ ; Chen, Xueqiu ¹ ; Yang, Yimin ¹ ; Pan, Lingtao ¹ ; Du, Zhendong ¹ ; Sun, Hongchao ³ ; Yao, Chaoqun ⁴ ; Ma, Guangxu ¹ ; Du, Aifang ¹ ;

作者机构： 1.Zhejiang Univ, Coll Anim Sci, Zhejiang Prov Key Lab Prevent Vet Med, Inst Prevent Vet Med, Hangzhou, Zhejiang, Peoples R China

2.Zhejiang Univ, Hainan Inst, Sanya, Peoples R China

3.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Med, Dept Anim Parasitol, Hangzhou, Zhejiang, Peoples R China

4.Ross Univ, Dept Biomed Sci & One Hlth Ctr Zoonoses & Trop Ve, Sch Vet Med, Basseterre, St Kitts & Nevi

5.Ross Univ, One Hlth Ctr Zoonoses & Trop Vet Med, Sch Vet Med, Basseterre, St Kitts & Nevi

6.Univ Melbourne, Melbourne Vet Sch, Dept Vet Biosci, Parkville, Vic, Australia

关键词： Toxoplasma gondii; PP2Ac alpha; B '/PR61 subunit; amylopectin metabolism; virulence

期刊名称：MICROBIOLOGY SPECTRUM （ 影响因子：3.7； 五年影响因子：5.9 ）

ISSN： 2165-0497

年卷期： 2023 年 11 卷 3 期

页码：

收录情况： SCI

摘要： Here, we report that the inhibition of the PP2A subfamily by okadaic acid results in an accumulation of polysaccharides in the acute infection stage (tachyzoites) of Toxoplasma gondii, which is a protozoan of global zoonotic importance and a model for the apicomplexan parasites. The loss of the catalytic subunit alpha of PP2A (Delta PP2Ac alpha) in RH Delta ku80 leads to the polysaccharide accumulation phenotype in the base of tachyzoites as well as residual bodies and significantly compromises the intracellular growth in vitro and the virulence in vivo. A metabolomic analysis revealed that the accumulated polysaccharides in Delta PP2Ac alpha are derived from interrupted glucose metabolism, which affects the production of ATP and energy homeostasis in the T. gondii knockout. The assembly of the PP2Ac alpha holoenzyme complex involved in the amylopectin metabolism in tachyzoites is possibly not regulated by LCMT1 or PME1, and this finding contributes to the identification of the regulatory B subunit (B/PR61). The loss of B '/PR61 results in the accumulation of polysaccharide granules in the tachyzoites as well as reduced plaque formation ability, exactly the same as Delta PP2Ac alpha. Taken together, we have identified a PP2Ac alpha-B '/PR61 holoenzyme complex that plays a crucial role in the carbohydrate metabolism and viability in T. gondii, and its deficiency in function remarkably suppresses the growth and virulence of this important zoonotic parasite both in vitro and in vivo. Hence, rendering the PP2Ac alpha-B '/PR61 holoenzyme functionless should be a promising strategy for the intervention of Toxoplasma acute infection and toxoplasmosis. IMPORTANCE Toxoplasma gondii switches back and forth between acute and chronic infections, mainly in response to host immunologic status, which is characterized by flexible but specific energy metabolism. Polysaccharide granules are accumulated in the acute infection stage of T. gondii that have been exposed to a chemical inhibitor of the PP2A subfamily. The genetic depletion of the catalytic subunit alpha of PP2A leads to this phenotype and significantly affects the cell metabolism, energy production, and viability. Further, a regulatory B subunit PR61 is necessary for the PP2A holoenzyme to function in glucose metabolism and in the intracellular growth of T. gondii tachyzoites. A deficiency of this PP2A holoenzyme complex (PP2Ac alpha-B '/PR61) in T. gondii knockouts results in the abnormal accumulation of polysaccharides and the disruption of energy metabolism, suppressing their growth and virulence. These findings provide novel insights into cell metabolism and identify a potential target for an intervention against a T. gondii acute infection.