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Expression level of a gibberellin 20-oxidase gene is associated with multiple agronomic and quality traits in barley

文献类型： 外文期刊

作者： Jia, Qiaojun 1 ; Zhang, Xiao-Qi 3 ; Westcott, Sharon 2 ; Broughton, Sue 2 ; Cakir, Mehmet 3 ; Yang, Jianming 1 ; Lance 1 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Crop & Nucl Technol Utilizat, Hangzhou, Zhejiang, Peoples R China

2.Govt Western Australia, Dept Agr & Food, S Perth, WA 6155, Australia

3.Murdoch Univ, Western Australian Agr Biotechnol Ctr, Perth, WA, Australia

关键词： quantitative trait locus: expression;gene expression marker: GEM;quality trait;hectolitre weight

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

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收录情况： SCI

摘要： The use of dwarfing genes has resulted in the most significant improvements in yield and adaptation in cereal crops. The allelic dwarfing gene sdw1/denso has been used throughout the world to develop commercial barley varieties. The sdw1 gene has never been used successfully for malting barley, but only for a large number of feed varieties. One of the gibberellin 20-oxidase genes (Hv20ox (2) ) was identified as the candidate gene for sdw1/denso. Semi-quantitative real-time RT-PCR revealed that Hv20ox (2) was expressed at different levels in various organs of barley. Transcriptional levels were reduced in leaf blade, sheath, stem and rachis tissue in the barley variety Baudin with the denso gene. Subsequently, the relative expression levels of Hv20ox (2) were determined by quantitative real-time RT-PCR in a doubled haploid population and mapped as a quantitative trait. A single expression quantitative trait locus (eQTL) was identified and mapped to its structural gene region on chromosome 3H. The eQTL was co-located with QTLs for yield, height, development score, hectolitre weight and grain plumpness. The expression level of Hv20ox (2) was reduced fourfold in the denso mutant, but around 60-fold in the sdw1 mutant, compared to the control variety. The reduced expression level of Hv20ox (2) enhanced grain yield by increasing the number of effective tillers, but had negative effects on grain and malting quality. The sdw1 gene can be used only in feed barley due to its severe reduction of Hv20ox (2) expression. The gene expression marker for Hv20ox (2) can be used to distinguish different alleles of sdw1/denso.

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