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Characterization of microRNAs from sheep (Ovis aries) using computational and experimental analyses

文献类型： 外文期刊

作者： Sheng, Xihui 1 ; Song, Xuemei 3 ; Yu, Yan 4 ; Niu, Lili 5 ; Li, Shangang 6 ; Li, Hongbin 7 ; Wei, Caihong 1 ; Liu, Tao 1 ; Z 1 ;

作者机构： 1.Chinese Acad Agr Sci, Natl Ctr Mol Genet & Breeding Anim, Inst Anim Sci, Beijing 100193, Peoples R China

2.Cornell Univ, Coll Vet Med, Ithaca, NY 14853 USA

3.ZheJiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Zhejiang, Peoples R China

4.Shandong Univ Tradit Chinese Med, Reprod Med Ctr, Hosp Affiliated 2, Jinan 250001, Peoples R China

5.Gyeongsang Natl Univ, Div Appl Life Sci, Grad Sch, Jinju 660701, South Korea

6.Shanghai Jiao Tong Univ, Dept Lab Anim Sci, Sch Med, Shanghai 200025, Peoples R China

7.Chinese Acad Sci, Inst Zool, Beijing 100101, Peoples R China

关键词： genes;cDNA library;small RNA;microRNA-3p: miRNA-3p;expression;characterization;microRNA-5p: miRNA-5p;expression;characterization;muscle development

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN：

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收录情况： SCI

摘要： Ovis aries is one of the most important agricultural livestock for meat production, and also is an ideal model organism for biological and comparative genomics studies. Many miRNAs have been reported for their important roles in developmental processes in various animals, but there is limited information about O. aries miRNAs. In this study, combining a computational method based on expressed sequence tag (EST) analysis with experimental identification based on small RNA cDNA library, we identified 31 miRNAs belong to 24 families in sheep, 2 of which were novel miRNAs which had never been previously identified in any species. Especially, we cloned 12 miRNAs from the sheep skeletal muscle, which were good candidate miRNAs to be studied about the miRNA-dependant regulated process of muscle development, and we identified four pairs of miRNA/miRNA* and one pair of miRNA-3p/miRNA-5p from sheep EST sequences. Expression analysis indicated that some miRNAs were expressed in a specific tissue, and the pair of miRNA-3p/miRNA-5p and one pair of miRNA/miRNA* had a similar relative expression pattern in some tissues, respectively. Further, we predicted 120 potential target genes of 31 oar-miRNAs on the 3'UTR of O. aries genes. Gene ontology analysis showed that most of these genes took part in the cellular process and metabolic process. Our results enriched the O. aries miRNA database and provided useful information for investigating biological functions of miRNAs and miRNA* in sheep.

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