浙江省农业科学院机构知识库

Expression of the hIGF-I gene driven by the Fhx/P25 promoter in the silk glands of germline silkworm and transformed BmN cells

收藏
分享
全文链接

文献类型：外文期刊

作者： Li, Yanmei ¹ ; Cao, Guangli ¹ ; Wang, Yang ¹ ; Xue, Renyu ¹ ; Zhou, Wenlin ³ ; Gong, Chengliang ¹ ;

作者机构： 1.Soochow Univ, Sch Biol & Basic Med Sci, Suzhou 215123, Peoples R China

2.Soochow Univ, Natl Engn Lab Modern Silk, Suzhou 215123, Peoples R China

3.Zhejiang Acad Agr Sci, Sericulture Res Inst, Hangzhou 310021, Zhejiang, Peoples R China

4.Soochow Univ, Preclin Med & Biol Sci Coll, Suzhou 215123, Peoples R China

关键词： BmN cell;Bombyx mori;Fhx/P25 promoter;hIGF-I;Silk gland;Transgenic silkworm

期刊名称：BIOTECHNOLOGY LETTERS （影响因子：2.461；五年影响因子：2.457 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The expression of the human insulin-like growth factor (hIGF-I) gene driven by the Fhx/P25 promoter in the silk glands of transgenic silkworms (Bombyx mori) and in transformed silkworm cells, was achieved using BmN cells transfected with a piggyBac vector, pigA3GFP-Fhx/P25-hIGF-ie-neo containing a neomycin-resistance gene (neo), a green fluorescent protein gene (gfp), an hIGF-I gene, and a helper plasmid containing the piggyBac transposase sequence under the control of the B. mori actin 3 (A3) promoter. We selected stably transformed BmN cells expressing hIGF-I using the antibiotic G418. The expression level of hIGF-I was about 450pg in 310 super(6) cells, determined by ELISA. The piggyBac vector was transferred into the silkworm eggs using sperm-mediated gene transfer. The expression level of hIGF-I per gram fresh posterior silk glands of G4 transgenic silkworms was approx. 150ng.

相关文献

[1]Expression of the B Subunit of Escherichia coli Heat-Labile Enterotoxin in Transformed Bombyx mori BmN Cells. Zhou, Wen-Lin,Cao, Jin-Ru,Ye, Ai-Hong,Weng, Hong-Biao,He, Li-Hua,Wang, Yong-Qiang,Gong, Cheng-Liang,Xue, Ren-Yu,Cao, Guang-Li,Gong, Cheng-Liang,Xue, Ren-Yu,Cao, Guang-Li. 2012

[2]Expression of hGM-CSF in silk glands of transgenic silkworms using gene targeting vector. Xue, Renyu,Cao, Guangli,Gong, Chengliang,Xue, Renyu,Chen, Huimei,Cui, Linlin,Cao, Guangli,Zheng, Xiaojian,Gong, Chengliang,Zhou, Wenlin. 2012

[3]Proteome analysis of silk gland proteins from the silkworm, Bombyx mori. Zhang, PB,As, YC,Yamamoto, K,Banno, Y,Wang, YQ,Tsuchida, K,Kawaguchi, Y,Fujii, H.

[4]Reducing blood glucose level in TIDM mice by orally administering the silk glands of transgenic hIGF-I silkworms. Cong, Liang,Cao, Guangli,Xue Renyu,Pan Zhonghua,Zheng Xiaojian,Gong, Chengliang,Cao, Guangli,Xue Renyu,Pan Zhonghua,Gong, Chengliang,Zhou, Wenlin. 2011

[5]Lowering the Blood Glucose of Diabetes Mellitus Mice by Oral Administration with Transgenic Human Insulin-like Growth Factor I Silkworms. Xue, Renyu,Wang, Yang,Cao, Guangli,Pan, Zhonghua,Zheng, Xiaojian,Gong, Chengliang,Xue, Renyu,Cao, Guangli,Pan, Zhonghua,Gong, Chengliang,Zhou, Wenlin.

[6]Systematic identification of mitochondrial lysine succinylome in silkworm (Bombyx mori) midgut during the larval gluttonous stage. Chen, Jine,Li, Fengbo,Liu, Yan,Shen, Weifeng,Du, Xin,He, Lihua,Meng, Zhiqi,Wang, Yongqiang,Ma, Xianliang. 2018

[7]Molecular and biochemical characterization of manganese-containing superoxide dismutase from the silkworm, Bombyx mori. Yamamoto, K,Zhang, PB,He, NJ,Wang, YQ,Aso, Y,Banno, Y,Fujii, H. 2005

[8]Heterologous expression characteristics of trichoderma viride endoglucanase V in the Silkworm, Bombyx mori L.. Miao, Yun-gen,Li, Xing-hua,Wang, Mei-xian,Zhang, Peng,Hu, Jia-biao,Sun, Chun-guang,Zhou, Fang,Niu, Yan-shan,Malik, Firdose Ahmad,Bhaskar, Roy,Yang, Hua-jun,Miao, Yun-gen,Liu, Xin-ju.

[9]The complete mitochondrial genome of Bombyx mori strain Baiyun (Lepidoptera: Bombycidae). Zhang, Huixian,Zhang, Huixian,Li, Fengbo,Meng, Zhiqi,Zhu, Xinrong.

[10]CLONING AND EXPRESSION PATTERN OF 3-DEHYDROECDYSONE 3 beta-REDUCTASE (3DE 3 beta-REDUCTASE) FROM THE SILKWORM, Bombyx mori L.. Miao, Yun-gen,Liu, Xin-ju.

[11]Proteome analysis of silkworm, bombyx mori, larval gonads: Characterization of proteins involved in sexual dimorphism and gametogenesis. Chen, Jin-e,Li, Jian-ying,You, Zheng-ying,Jiang, Zhen-dong,Zhong, Bo-xiong,Chen, Jin-e,Li, Jian-ying,Liu, Li-li,Chen, Ming,Liang, Jian-she,Ma, Ying-ying,Zhang, Hua-rong.

[12]Complete mitochondrial genome of a hybrid strain of the domesticated silkworm (Qiufeng x Baiyu). Li, Fengbo,Zhang, Huixian,Meng, Zhiqi,Zhang, Huixian,Liu, Peigang,Wang, Yongqiang.

[13]Comparison of Transformation Efficiency of piggyBac Transposon among Three Different Silkworm Bombyx mori Strains. Zhong, Boxiong,Li, Jianying,Chen, Jin'e,Ye, Jian,Yu, Songdong.

[14]Cloning and Alternative Splicing Analysis of Bombyx mori Transformer-2 Gene using Silkworm EST Database. Niu, BL,Meng, ZQ,Tao, YZ,Lu, SL,Weng, HB,He, LH,Shen, WF.

作者其他论文更多>>

3D-based precise evaluation pipeline for maize ear rot using multi-view stereo reconstruction and point cloud semantic segmentation

作者：Yang, Rui;He, Yong;Lu, Xiangyu;Liu, Fei;Zhao, Yiying;Li, Yanmei;Yang, Yinhui;Kong, Wenwen

关键词：Maize ear rot; Multi-view stereo reconstruction; Point cloud; 3D semantic segmentation; Deep learning
Effects of short-term florfenicol exposure on the gene expression pattern, midgut microbiota, and metabolome in the lepidopteran model silkworm (Bombyx mori)

作者：Zhang, Xing;Huo, Yiming;Kong, Yifei;Qin, Feiju;Hu, Xiaolong;Zhou, Wenlin

关键词：Silkworm; Florfenicol; Microbiota; Metabolites; Gene expression
Pathogen resistance in soils associated with bacteriome network reconstruction through reductive soil disinfestation

作者：Zhu, Weijing;Lu, Xiaolin;Hong, Chunlai;Hong, Leidong;Zhu, Fengxiang;Yao, Yanlai;Ding, Jian;Zhou, Wenlin

关键词：Reductive soil disinfestation; Bacteriome network; Keystone taxa; Sensitive OTU; Fungistasis
Dietary administration with hydrolyzed silk sericin improves the intestinal health of diabetic rats

作者：Zhou, Wenlin;Ye, Aihong;Weng, Yujie;Liu, Qian;Wang, Chonglong;Zhang, Yu-Qing;Zhang, Xing

关键词：sericin; type II diabetes; gut microbiota; health; rats
The effects of short-term dietary exposure to SiO2 nanoparticle on the domesticated lepidopteran insect model silkworm (Bombyx mori): Evidence from the perspective of multi-omics

作者：Zhang, Xing;Shao, Wenjing;Huo, Yiming;Kong, Yifei;Qin, Fenju;Zhang, Wenxue;Li, Song;Hu, Xiaolong;Zhou, Wenlin;Wu, Xuehui

关键词：Silkworm; nSiO2; Dietary exposure; Microbial diversity; Metabolites
The effects of polylactic acid bioplastic exposure on midgut microbiota and metabolite profiles in silkworm (Bombyx mori): An integrated multi-omics analysis

作者：Wu, Xuehui;Chen, Xuedong;Cao, Jinru;Zhou, Wenlin;Zhang, Xing;Hu, Xiaolong

关键词：Silkworm; Polylactic acid; Microbiota; Metabolite
Short-term triphenyltin exposure alters microbial homeostasis in the silkworm (Bombyx mori) midgut

作者：Zhou, Wenlin;Chen, Xuedong;Wu, Xuehui;Ye, Aihong;Cao, Jinru;Zhang, Xing;Hu, Xiaolong

关键词：

Expression of the hIGF-I gene driven by the Fhx/P25 promoter in the silk glands of germline silkworm and transformed BmN cells

作者其他论文更多>>

3D-based precise evaluation pipeline for maize ear rot using multi-view stereo reconstruction and point cloud semantic segmentation

Effects of short-term florfenicol exposure on the gene expression pattern, midgut microbiota, and metabolome in the lepidopteran model silkworm (Bombyx mori)

Pathogen resistance in soils associated with bacteriome network reconstruction through reductive soil disinfestation

Dietary administration with hydrolyzed silk sericin improves the intestinal health of diabetic rats

The effects of short-term dietary exposure to SiO2 nanoparticle on the domesticated lepidopteran insect model silkworm (Bombyx mori): Evidence from the perspective of multi-omics

The effects of polylactic acid bioplastic exposure on midgut microbiota and metabolite profiles in silkworm (Bombyx mori): An integrated multi-omics analysis

Short-term triphenyltin exposure alters microbial homeostasis in the silkworm (Bombyx mori) midgut

意见箱

Expression of the hIGF-I gene driven by the Fhx/P25 promoter in the silk glands of germline silkworm and transformed BmN cells

作者其他论文 更多>>

3D-based precise evaluation pipeline for maize ear rot using multi-view stereo reconstruction and point cloud semantic segmentation

Effects of short-term florfenicol exposure on the gene expression pattern, midgut microbiota, and metabolome in the lepidopteran model silkworm (Bombyx mori)

Pathogen resistance in soils associated with bacteriome network reconstruction through reductive soil disinfestation

Dietary administration with hydrolyzed silk sericin improves the intestinal health of diabetic rats

The effects of short-term dietary exposure to SiO2 nanoparticle on the domesticated lepidopteran insect model silkworm (Bombyx mori): Evidence from the perspective of multi-omics

The effects of polylactic acid bioplastic exposure on midgut microbiota and metabolite profiles in silkworm (Bombyx mori): An integrated multi-omics analysis

Short-term triphenyltin exposure alters microbial homeostasis in the silkworm (Bombyx mori) midgut

意 见 箱

作者其他论文更多>>

意见箱