A rapid on-site visualization platform based on RPA coupled with CRISPR-Cas12a for the detection of genetically modified papaya 'Huanong No.1'
文献类型： 外文期刊
作者： Zhu, Lili 1 ; He, Gongwen 1 ; Yang, Guiqin 1 ; Yang, Wenli 1 ; He, Ying 1 ; Chen, Jian 1 ; Chen, Yanxin 1 ; Ji, Yi 2 ; Pan, Zhiwen 1 ; Yao, Juan 1 ; Chen, Xiaoyun 2 ; Jiang, Dagang 1 ;
作者机构： 1.South China Agr Univ, Coll Life Sci, Guangdong Prov Key Lab Prot Funct & Regulat Agr Or, Guangzhou 510642, Peoples R China
2.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Key Lab Traceabil Agr Genet Modified Organisms, Minist Agr & Rural Affairs, Hangzhou 310021, Peoples R China
关键词： Huanong No.1; RPA; CRISPR-Cas12a; Visualization; GM papaya
期刊名称：TALANTA （ 影响因子：6.1； 五年影响因子：5.5 ）
ISSN： 0039-9140
年卷期： 2024 年 277 卷
页码：
收录情况： SCI
摘要： The Papaya ringspot virus (PRSV)-resistant genetically modified (GM) papaya 'Huanong No.1' has been certified as safe for consumption and widely planted in China for about 18 years. To protect consumers' rights and facilitate government supervision and monitoring, it is necessary to establish a simple, rapid, and specific detection method for 'Huanong No.1'. Herein, we developed a platform based on recombinase polymerase amplification (RPA) coupled with CRISPR-Cas12a for the detection of 'Huanong No.1'. The RPA-CRISPR-Cas12a platform was found to have high specificity, with amplification signals only present in 'Huanong No.1'. Additionally, the platform was highly sensitive, with a limit of detection (LOD) of approximately 20 copies. The detection process was fast and could be completed in less than 1 h. This novel platform enables the rapid on-site visualization detection of 'Huanong No.1', eliminating dependence on laboratory conditions and specialized instruments, and can serve as a technical reference for the rapid detection of other GM plants.
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