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Edwardsiella piscicida YccA: A novel virulence factor essential to membrane integrity, mobility, host infection, and host immune response

文献类型： 外文期刊

作者： Jin, Mengru 1 ; He, Jiaojiao 2 ; Li, Jun 4 ; Hu, Yonghua 2 ; Sun, Dongmei 1 ; Gu, Hanjie 2 ;

作者机构： 1.Heilongjiang Bayi Agr Univ, Coll Life Sci & Technol, Daqing 163319, Peoples R China

2.CATAS, Hainan Acad Trop Agr Resource, Inst Trop Biosci & Biotechnol, Haikou 571101, Hainan, Peoples R China

3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China

4.Lake Super State Univ, Sch Sci & Med, Sault Sainte Marie, MI 49783 USA

5.Hainan Univ, Sch Life Sci, Haikou 570228, Hainan, Peoples R China

6.Hainan Prov Key Lab Funct Components Res & Utiliz, Haikou 571101, Hainan, Peoples R China

关键词： Edwardsiella piscicida; YccA; Adversity; Virulence; Infection; Tilapia; Host immune response

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.622； 五年影响因子：4.799 ）

ISSN： 1050-4648

年卷期： 2022 年 126 卷

页码：

收录情况： SCI

摘要： YccA is a hydrophobic protein with seven transmembrane domains. The function of YccA is largely unknown in pathogenic bacteria. Edwardsiella piscicide (formerly known as E. tarda) is an aquatic pathogen that can infect various economically important fish, including flounder (Paralichthys olivaceus) and tilapia (Oreochromis niloticus). In this study, we investigated the role of YccA in E. piscicida by the construction of a mar kerless yccA in-frame mutant strain, TX01 Delta yccA. We found that (i) in comparison to the wild type TX01, TX01 Delta yccA exhibited markedly compromised tolerance to high temperature and tobramycin; (ii) deletion of yccA significantly impaired the integrity of the cell membrane and retarded bacterial biofilm formation and mobility; (iii) deficiency of yccA reduced bacterial adhesion and invasion of fish cells and immune tissues, while the introduction of a trans-expressed yccA gene restored the lost virulence of TX01 Delta yccA; and (iv) host immune responses induced by TX01 and TX01 Delta yccA were different in terms of reactive oxygen species (ROS) levels and expression levels of cytokines. Taken together, the results of our study indicate that YccA is a novel virulence factor of E. piscicida, and YccA is essential for bacterial pathogenicity through evasion of the host's innate immune functions.

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