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Evolutionary gain of oligosaccharide hydrolysis and sugar transport enhanced carbohydrate partitioning in sweet watermelon fruits

文献类型： 外文期刊

作者： Ren, Yi 1 ; Li, Maoying 1 ; Guo, Shaogui 1 ; Sun, Honghe 1 ; Zhao, Jianyu 4 ; Zhang, Jie 1 ; Liu, Guangmin 1 ; He, Hongju 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Natl Watermelon & Melon Improvement Ctr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China

2.Lawrence Berkeley Natl Lab, Joint BioEnergy Inst, Berkeley, CA 94720 USA

3.Lawrence Berkeley Natl Lab, Biol Syst & Engn Div, Berkeley, CA 94720 USA

4.China Agr Univ, Beijing Key Lab Growth & Dev Regulat Protected Ve, MOE Joint Int Res Lab Crop Mol Breeding, Beijing 100193, Peoples R China

5.Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany

6.Ctr Plant Syst Biol & Biotechnol, Plovdiv 4000, Bulgaria

7.Univ Calif Berkeley, Dept Plant & Microbial Biol, Berkeley, CA 94720 USA

期刊名称：PLANT CELL （ 影响因子：11.277； 五年影响因子：12.061 ）

ISSN： 1040-4651

年卷期： 2021 年 33 卷 5 期

页码：

收录情况： SCI

摘要： How raffinose (Raf) family oligosaccharides, the major translocated sugars in the vascular bundle in cucurbits, are hydrolyzed and subsequently partitioned has not been fully elucidated. By performing reciprocal grafting of watermelon (Citrullus lanatus) fruits to branch stems, we observed that Raf was hydrolyzed in the fruit of cultivar watermelons but was backlogged in the fruit of wild ancestor species. Through a genome-wide association study, the alkaline alpha-galactosidase ClAGA2 was identified as the key factor controlling stachyose and Raf hydrolysis, and it was determined to be specifically expressed in the vascular bundle. Analysis of transgenic plants confirmed that ClAGA2 controls fruit Raf hydrolysis and reduces sugar content in fruits. Two single-nucleotide polymorphisms (SNPs) within the ClAGA2 promoter affect the recruitment of the transcription factor ClNF-YC2 (nuclear transcription factor Y subunit C) to regulate ClAGA2 expression. Moreover, this study demonstrates that C. lanatus Sugars Will Eventually Be Exported Transporter 3 (ClSWEET3) and Tonoplast Sugar Transporter (ClTST2) participate in plasma membrane sugar transport and sugar storage in fruit cell vacuoles, respectively. Knocking out ClAGA2, ClSWEET3, and ClTST2 affected fruit sugar accumulation. Genomic signatures indicate that the selection of ClAGA2, ClSWEET3, and ClTST2 for carbohydrate partitioning led to the derivation of modern sweet watermelon from non-sweet ancestors during domestication.

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