An Agrobacterium-mediated non-antibiotic selection-based transformation system for rice (Oryza sativa ssp. indica) cultivar "93-11" successfully produces TAC1-silenced transgenic plants
文献类型： 外文期刊
作者： Hou, Jingjing 1 ; Chen, Hao 2 ; Fang, Yuzhen 1 ; Zhu, Ying 1 ; Han, Bing 1 ; Sun, Chuanqing 1 ; Fu, Yongcai 1 ;
作者机构： 1.China Agicultural Univ, Natl Ctr Evaluat Agr Wild Plants Rice, Dept Plant Genet & Breeding, MOE Key Lab Crop Heterosis & Utilizat,Beijing Key, Beijing 100193, Peoples R China
2.Fujian Acad Agr Sci, Fujian Engn Res Ctr Characterist Upland Crops Bre, Inst Crop Sci, Fujian Engn Lab Crop Mol Breeding, Fuzhou 350013, Peoples R China
关键词： Agrobacterium-mediated transformation system; 93-11; TAC1
期刊名称：IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT （ 影响因子：2.252； 五年影响因子：2.139 ）
ISSN： 1054-5476
年卷期： 2021 年 57 卷 5 期
页码：
收录情况： SCI
摘要： The lice (Oryza saliva L. ssp. indica) cultivar "93-11" is a reference genotype used in many studies; however, its recalcitrance during transformation and regeneration greatly limits functional genomics and breeding research. In this study. we developed an efficient Agrobacterium tumefaciens-mediated transformation system for "93-11", based on the phosphor mannose isomerase (PMI) positive selection system. Calli of "93-11" were transformed with the Agrobacterium strain EHA105 harboring a binary vector, containing the PMI gene and an RNAi sequence targeting TILLER ANGLE CONTROLLING 1 (TACI). We also developed a method for removing Agrobacterium from the callus following co-cultivation and determined the optimal conditions for PMI selection and callus differentiation. Compared with the hygromycin phosphotransferase (HPT) selection system, newly generated calli were recovered at higher rates on the PMI selection medium, with "93-11" transformation frequency reaching 7.50%. Notably, the down-regulation of TACI in the "93-11" genetic background led to a more compact plant architecture. Overall, this work presents a genetic transformation system suitable for the reference indica variety "93-11" using a nonantibiotic selectable agent. This advance will facilitate functional genomic research and the improvement of agronomic traits for indica varieties recalcitrant to transformation.
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