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Identification of Wheat Glutamate Synthetase Gene Family and Expression Analysis under Nitrogen Stress

文献类型： 外文期刊

作者： Li, Songshuo 1 ; Jiao, Bo 2 ; Wang, Jiao 2 ; Zhao, Pu 2 ; Dong, Fushuang 2 ; Yang, Fan 2 ; Ma, Chunhong 2 ; Guo, Peng 1 ; Zhou, Shuo 2 ;

作者机构： 1.Hebei Univ Sci & Technol, Sch Biol Sci & Engn, Yuxiang St 26, Shijiazhuang 050018, Peoples R China

2.Hebei Acad Agr & Forestry Sci, Inst Biotechnol & Food Sci, Hebei Key Lab Plant Genet Engn, Shijiazhuang 050051, Peoples R China

关键词： glutamate synthase; wheat; N stress; tissue specificity

期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.3 ）

ISSN：

年卷期： 2024 年 15 卷 7 期

页码：

收录情况： SCI

摘要： Nitrogen (N), as the main component of biological macromolecules, maintains the basic process of plant growth and development. GOGAT, as a key enzyme in the N assimilation process, catalyzes alpha-ketoglutaric acid and glutamine to form glutamate. In this study, six GOGAT genes in wheat (Triticum aestivum L.) were identified and classified into two subfamilies, Fd-GOGAT (TaGOGAT2s) and NADH-GOGAT (TaGOGAT3s), according to the type of electron donor. Subcellular localization prediction showed that TaGOGAT3-D was localized in mitochondria and that the other five TaGOGATs were localized in chloroplasts. Via the analysis of promoter elements, many binding sites related to growth and development, hormone regulation and plant stress resistance regulations were found on the TaGOGAT promoters. The tissue-specificity expression analysis showed that TaGOGAT2s were mainly expressed in wheat leaves and flag leaves, while TaGOGAT3s were highly expressed in roots and leaves. The expression level of TaGOGATs and the enzyme activity of TaGOGAT3s in the leaves and roots of wheat seedlings were influenced by the treatment of N deficiency. This study conducted a systematic analysis of wheat GOGAT genes, providing a theoretical basis not only for the functional analysis of TaGOGATs, but also for the study of wheat nitrogen use efficiency (NUE).

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