Lasiodiplodia theobromae protein LtScp1 contributes to fungal virulence and protects fungal mycelia against hydrolysis by grapevine chitinase
文献类型： 外文期刊
作者： Peng, Junbo 1 ; Li, Xinghong 1 ; Li, Yonghua 1 ; Zhang, Wei 1 ; Zhou, Ying 1 ; Yan, Jiye 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing Key Lab Environm Friendly Management Frui, Beijing 100097, Peoples R China
期刊名称：ENVIRONMENTAL MICROBIOLOGY （ 影响因子：5.476； 五年影响因子：6.82 ）
ISSN： 1462-2912
年卷期： 2022 年 24 卷 10 期
页码：
收录情况： SCI
摘要： The LysM proteins have been reported to be important for the virulence and host immunity suppression in herbaceous plant pathogens, whereas far less information is documented in the woody plant pathogen Lasiodiplodia theobromae. To investigate the functional mechanism of LysM protein in L. theobromae, one gene LtScp1 was cloned and characterized detailedly in the current study. Transcription profiling revealed that LtScp1 was highly expressed at the infectious stages. Compared to wild type, overexpression and silencing of LtScp1 in L. theobromae led to significantly increased and decreased lesion areas, respectively. Moreover, LtScp1 was determined to be a secreted protein via a yeast signal peptide trapping system. Interestingly, LtScp1 was confirmed to be modified by the N-glycosylation, which is necessary for the homodimerization of LtScp1 molecules. Furthermore, it was found that LtScp1 interacted with the grapevine chitinase VvChi4 and interfered the ability of VvChi4 to bind chitin. Collectively, these results suggest that LtScp1 functions as a virulence factor to protect the fungus from degradation during the infection.
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