Lasiodiplodia theobromae protein LtScp1 contributes to fungal virulence and protects fungal mycelia against hydrolysis by grapevine chitinase
文献类型： 外文期刊
作者： Peng, Junbo 1 ; Li, Xinghong 1 ; Li, Yonghua 1 ; Zhang, Wei 1 ; Zhou, Ying 1 ; Yan, Jiye 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Inst Plant Protect, Beijing Key Lab Environm Friendly Management Frui, Beijing 100097, Peoples R China
期刊名称：ENVIRONMENTAL MICROBIOLOGY （ 影响因子：5.476； 五年影响因子：6.82 ）
ISSN： 1462-2912
年卷期： 2022 年 24 卷 10 期
页码：
收录情况： SCI
摘要： The LysM proteins have been reported to be important for the virulence and host immunity suppression in herbaceous plant pathogens, whereas far less information is documented in the woody plant pathogen Lasiodiplodia theobromae. To investigate the functional mechanism of LysM protein in L. theobromae, one gene LtScp1 was cloned and characterized detailedly in the current study. Transcription profiling revealed that LtScp1 was highly expressed at the infectious stages. Compared to wild type, overexpression and silencing of LtScp1 in L. theobromae led to significantly increased and decreased lesion areas, respectively. Moreover, LtScp1 was determined to be a secreted protein via a yeast signal peptide trapping system. Interestingly, LtScp1 was confirmed to be modified by the N-glycosylation, which is necessary for the homodimerization of LtScp1 molecules. Furthermore, it was found that LtScp1 interacted with the grapevine chitinase VvChi4 and interfered the ability of VvChi4 to bind chitin. Collectively, these results suggest that LtScp1 functions as a virulence factor to protect the fungus from degradation during the infection.
- 相关文献
作者其他论文 更多>>
-
Gene expression profiles of Chinese medaka ( Oryzias sinensis ) primary hepatocytes in response to estrone (E1 ), 17 i3-estradiol (E2 ) and estriol (E3 )
作者：Wang, Yue;Lu, Junhui;Xie, Zhongtang;Huai, Narma;Zhang, Kailun;Zhou, Ying;Reze, Yilihamu;Li, Xiqing;Zhang, Zhaobin;Zhu, Hua
关键词：Oryzias sinensis; Primary hepatocytes; Natural estrogens; Vitellogenin; toxicogenomics
-
Characterization of Fungal Pathogens Causing Blueberry Fruit Rot Disease in China
作者：Zhou, Yueyan;Zhang, Wei;Wu, Linna;Chen, Pengzhao;Li, Xinghong;Yan, Jiye;Zhou, Yueyan;Chethana, Kandawatte Wedaralalage Thilini;Hyde, Kevin D.;Zhou, Yueyan;Tangtrakulwanich, Khanobporn;Chethana, Kandawatte Wedaralalage Thilini;Wen, Guangqin;Al-Otibi, Fatimah;Hyde, Kevin D.
关键词：
Vaccinium spp.; new host records; phylogenetic analysis; pathogenicity -
Chromosome-level genome assembly of tetraploid Chinese cherry (Prunus pseudocerasus)
作者：Zhang, Wei;Wang, Xuncheng;Peng, Junbo;Xing, Qikai;Yan, Jiye;Wang, Jing;Duan, Xuwei;Zhang, Xiaoming;Zhang, Kaichun
关键词：
-
An Integrated Rapid Detection of Botryosphaeriaceae Species in Grapevine Based on Recombinase Polymerase Amplification, CRISPR/Cas12a, and Lateral Flow Dipstick
作者：Wang, Baoyu;Fan, Anran;Liu, Mei;Zhou, Ying;Zhang, Wei;Yan, Jiye
关键词：Botryosphaeria dieback; field detection; LFD; RPA-CRISPR/Cas12a
-
Argonaute1-Dependent LtmilR2 Negatively Regulated Infection of Lasiodiplodia theobromae by Targeting a Guanine Nucleotide Exchange Factor in RAS Signalling
作者：Huang, Caiping;He, Yuanyuan;Zhang, Wei;Wang, Jingpeng;Liu, Mei;Deepali, Borala Liyanage D.;Yan, Jiye
关键词：grapevine canker disease;
Lasiodiplodia theobromae ; milRNA; pathogenicity; RNA fungicide -
Transcriptomic analyses of LtEpg1-and VvKINβ1-transgenic plants in response to Lasiodiplodia theobromae infection
作者：Peng, Junbo;Wang, Xuncheng;Abeywickrama, Pranami D.;Guo, Hui;Liu, Mei;Xing, Qikai;Yan, Jiye
关键词：Lasiodiplodia theobromae; Nicotiana benthamiana; Transcriptome; Defence response
-
Three New Records of Pathogens Causing Stem Blight on Vaccinium corymbosum in China
作者：Zhou, Yueyan;Tangtrakulwanich, Khanobporn;Chethana, Kandawatte Wedaralalage Thilini;Zhou, Yueyan;Wu, Linna;Ren, Kaixuan;Wang, Meng;Wang, Nannan;Li, Xinghong;Zhang, Wei;Yan, Jiye;Zhou, Yueyan;Chethana, Kandawatte Wedaralalage Thilini;Hyde, Kevin D.
关键词：
Colletotrichum ;Curvularia ;Diaporthe ; morphology; phylogeny; Koch's postulates; new host records
