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Integrative Physiological and Transcriptomic Analysis Reveals Metabolic Adaptation and Cold-Tolerance Marker Development in Winter Rye Under Low-Temperature Stress

文献类型： 外文期刊

作者： Li, Haonan 1 ; Zhao, Jiahuan 1 ; He, Chenguang 1 ; Guan, Yang 2 ; Guan, Huimin 1 ; He, Ting 1 ; Meng, Dexu 1 ; Wang, Xiaoping 1 ; Tang, Yimiao 3 ;

作者机构： 1.Harbin Normal Univ, Coll Life Sci & Technol, Key Lab Mol Cell Genet & Genet Breeding Heilongjia, Harbin 150025, Peoples R China

2.Harbin Normal Univ, Coll Life Sci & Technol, Heilongjiang Prov Lab Plant Physiol, Harbin 150025, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Hybrid Wheat, Beijing Key Lab Mol Genet Hybrid Wheat, Beijing Key Lab Crop Mol Design & Intelligent Bree, Beijing 100097, Peoples R China

关键词： rye (Secale cereale); low-temperature stress; metabolism; molecular markers

期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）

ISSN： 2223-7747

年卷期： 2025 年 14 卷 11 期

页码：

收录情况： SCI

摘要： Rye (Secale cereale), a cereal crop with high cold tolerance, serves as an ideal model for investigating plant cold adaptation mechanisms. Despite recent progress in identifying numerous genes and metabolic changes associated with cold tolerance, the detailed regulatory networks and coordinated interactions between metabolic pathways under low-temperature stress in rye remain unclear. In this study, we focused on the winter rye variety "Winter" and systematically explored its metabolic regulatory responses to cold stress through a combination of low-temperature treatments, phenotypic observations, antioxidant enzyme activity assays, and transcriptomic analysis. Four rye varieties ("Winter", HZHM3, HZHM8, and "Victory") were compared for cold tolerance, with the results indicating that "Winter" and HZHM3 exhibit superior cold tolerance. Physiological analysis revealed that after 12 h of exposure to -4 degrees C, the activities of catalase (CAT), peroxidase (POD), and ascorbate peroxidase (APX) in "Winter" were significantly upregulated, displaying an initial increase followed by a decline over time. Transcriptomic sequencing identified 1643 differentially expressed genes (DEGs), and GO, KEGG, and GSEA enrichment analyses highlighted the critical roles of carbohydrate metabolism (ko00630) and amino acid metabolism (ko00250) pathways in the cold stress response. These pathways are interconnected through key metabolic intermediates such as L-glutamate, collectively regulating cold adaptation. Furthermore, based on the transcriptomic data, we identified and developed molecular markers associated with cold tolerance, detecting 10,846 EST-SSR and 250,116 EST-SNP markers. We successfully developed 13 EST-SSR primer pairs applicable to rye and 7 KASP markers. Notably, the KASP-665 marker effectively distinguishes between winter and spring rye, providing a reliable tool for marker-assisted selection in cold tolerance breeding. This study not only elucidates the metabolic regulatory mechanisms of rye under low-temperature stress but also provides a solid theoretical and technical foundation for future cold-tolerance breeding programs.

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