文献类型： 外文期刊
作者： Li, Xinran 1 ; Wang, Haoqian 2 ; Qi, Xin 1 ; Ji, Yi 3 ; Li, Fukai 1 ; Chen, Xiaoyun 3 ; Li, Kai 1 ; Li, Liang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China
2.Minist Agr & Rural Affairs, Dev Ctr Sci & Technol, Beijing 100176, Peoples R China
3.Zhejiang Acad Agr Sci, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310021, Peoples R China
关键词： RNA detection; PCR-free; biosensor; nanomaterial
期刊名称：BIOSENSORS-BASEL （ 影响因子：5.4； 五年影响因子：5.7 ）
ISSN：
年卷期： 2024 年 14 卷 4 期
页码：
收录情况： SCI
摘要： RNA is an important information and functional molecule. It can respond to the regulation of life processes and is also a key molecule in gene expression and regulation. Therefore, RNA detection technology has been widely used in many fields, especially in disease diagnosis, medical research, genetic engineering and other fields. However, the current RT-qPCR for RNA detection is complex, costly and requires the support of professional technicians, resulting in it not having great potential for rapid application in the field. PCR-free techniques are the most attractive alternative. They are a low-cost, simple operation method and do not require the support of large instruments, providing a new concept for the development of new RNA detection methods. This article reviews current PCR-free methods, overviews reported RNA biosensors based on electrochemistry, SPR, microfluidics, nanomaterials and CRISPR, and discusses their challenges and future research prospects in RNA detection.
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