Identification and expression profiling of GAPDH family genes involved in response to Sclerotinia sclerotiorum infection and phytohormones in Brassica napus
文献类型： 外文期刊
作者： Xu, Jing 1 ; Wang, Rongbo 3 ; Zhang, Xiong 4 ; Zhuang, Wei 1 ; Zhang, Yang 1 ; Lin, Jianxin 1 ; Zhan, Penglin 1 ; Chen, Shanhu 1 ; Lu, Heding 1 ; Wang, Airong 2 ; Liao, Changjian 1 ;
作者机构： 1.Fujian Acad Agr Sci, Fujian Germplasm Resources Ctr, Inst Crop Res, Fujian Prov Characterist Dry Crop Variety Breeding, Fuzhou, Peoples R China
2.Fujian Agr & Forestry Univ, State Key Lab Ecol Pest Control Fujian & Taiwan Cr, Fuzhou, Peoples R China
3.Inst Plant Protect, Fujian Acad Agr Sci, Fujian Key Lab Monitoring & Integrated Management, Fuzhou, Peoples R China
4.Oil Crops Res Inst, Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr & Rural Affairs PRC, Wuhan, Peoples R China
关键词： Brassica napus; GAPDH; gene family; Sclerotinia sclerotiorum; subcellular localization; nuclear translocation
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a crucial enzyme in glycolysis, an essential metabolic pathway for carbohydrate metabolism across all living organisms. Recent research indicates that phosphorylating GAPDH exhibits various moonlighting functions, contributing to plant growth and development, autophagy, drought tolerance, salt tolerance, and bacterial/viral diseases resistance. However, in rapeseed (Brassica napus), the role of GAPDHs in plant immune responses to fungal pathogens remains unexplored. In this study, 28 genes encoding GAPDH proteins were revealed in B. napus and classified into three distinct subclasses based on their protein structural and phylogenetic relationships. Whole-genome duplication plays a major role in the evolution of BnaGAPDHs. Synteny analyses revealed orthologous relationships, identifying 23, 26, and 26 BnaGAPDH genes with counterparts in Arabidopsis, Brassica rapa, and Brassica oleracea, respectively. The promoter regions of 12 BnaGAPDHs uncovered a spectrum of responsive elements to biotic and abiotic stresses, indicating their crucial role in plant stress resistance. Transcriptome analysis characterized the expression profiles of different BnaGAPDH genes during Sclerotinia sclerotiorum infection and hormonal treatment. Notably, BnaGAPDH17, BnaGAPDH20, BnaGAPDH21, and BnaGAPDH22 exhibited sensitivity to S. sclerotiorum infection, oxalic acid, hormone signals. Intriguingly, under standard physiological conditions, BnaGAPDH17, BnaGAPDH20, and BnaGAPDH22 are primarily localized in the cytoplasm and plasma membrane, with BnaGAPDH21 also detectable in the nucleus. Furthermore, the nuclear translocation of BnaGAPDH20 was observed under H2O2 treatment and S. sclerotiorum infection. These findings might provide a theoretical foundation for elucidating the functions of phosphorylating GAPDH.
- 相关文献
作者其他论文 更多>>
-
Dimethyl trisulfide (DMTS) fumigation enhances fruit quality and reduces postharvest incidence of bacterial canker in tomato
作者：Wang, Rongbo;Li, Benjin;Weng, Qiyong;Liu, Peiqing;Zhang, Qianrong;Liu, Peiqing
关键词：Bacterial canker of tomato; DMTS; Postharvest; Antioxidants; Ethylene biosynthesis
-
Rapid Visual Detection of Peronophythora litchii on Lychees Using Recombinase Polymerase Amplification Combined with Lateral Flow Assay Based on the Unique Target Gene Pl_101565
作者：Wang, Rongbo;Li, Benjin;Shi, Mingyue;Zhao, Yumei;Lin, Jinlong;Liu, Peiqing;Chen, Qinghe
关键词：Peronophythora litchii; recombinase polymerase amplification; lateral flow; lychee
-
Sepiolite-supported nanoscale zero-valent iron alleviates Cd&As accumulation in rice by reducing Cd&As bioavailability in paddy soil and promoting Cd&As sequestration in iron plaque
作者：Sun, Shuo;Zhang, Nan;Wang, Yanan;Zhang, Yang;Su, Shiming;Zeng, Xibai;Huang, Jiaqing;Wen, Jiong;Peng, Zhi
关键词：Sepiolite-supported nanoscale zero-valent iron; Cd&As co-contaminated soil; Cd&As bioavailability; Cd&As co-remediation; Iron plaque; Rice grain
-
A novel NAC transcription factor ZmNAC55 negatively regulates drought stress in Zea mays
作者：Fan, Kai;Wu, Yuchen;Mao, Zhijun;Yin, Kan;Pan, Xinfeng;Zhu, Xiaxiao;Jia, Qi;Li, Zhaowei;Liao, Changjian;Cui, Lili;Fan, Kai;Wu, Yuchen;Mao, Zhijun;Yin, Kan;He, Yuxi;Pan, Xinfeng;Jia, Qi;Li, Zhaowei
关键词：Maize; Drought stress; NAC transcription factor; ZmNAC55; Molecular mechanism
-
A robust high-throughput functional screening assay for plant pathogen effectors using the TMV-GFP vector
作者：Cao, Peng;Shi, Haotian;Zhang, Shuangxi;An, Yuyan;Zhang, Meixiang;Shi, Haotian;Chen, Jialan;Wang, Rongbo;Liu, Peiqing;Zhu, Yingfang
关键词：TMV-GFP; effector; virulence; avirulence; functional screening; technical advance
-
Bacillus velezensis stimulated the absorption of iodine to improve antioxidants and delay post-harvest senescence in microgreens
作者：Wang, Rongbo;Li, Benjin;Weng, Qiyong;Liu, Peiqing;Jin, Ting;Wang, Rongbo;Li, Benjin;Weng, Qiyong;Liu, Peiqing
关键词：Microgreens; Bacillus velezensis; Iodine; Antioxidants; Senescence
-
Transcriptome Analysis of Potential Regulatory Genes under Chemical Doubling in Maize Haploids
作者：Li, Youqiang;Pu, Rumin;Xiang, Wenqi;Meng, Xin;Yang, Shiqi;Hu, Gaojiao;Zhao, Shuang;Han, Jialong;Xia, Chao;Lan, Hai;Wang, Qingjun;Li, Jingwei;Lu, Yanli;Lin, Haijian;Zhan, Penglin;Liao, Changjian;Yu, Yongtao;Li, Gaoke
关键词：maize; haploid; transcriptome; mitosis; kinesin
