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MePP2C24, a cassava (Manihot esculenta) gene encoding protein phosphatase 2C, negatively regulates drought stress and abscisic acid responses in transgenic Arabidopsis thaliana

文献类型： 外文期刊

作者： Zeng, Jian 1 ; Wu, Chunlai 3 ; Ye, Xiaoxue 1 ; Zhou, Jiewei 1 ; Chen, Yingtong 1 ; Li, Lizhen 1 ; Lin, Man 1 ; Wang, Shuting 1 ; Liu, Siwen 1 ; Yan, Yan 2 ; Tie, Weiwei 2 ; Yang, Jinghao 2 ; Yan, Fei 6 ; Zeng, Liwang 2 ; Liu, Yujia 1 ; Hu, Wei 2 ;

作者机构： 1.Shaoguan Univ, Sch Biol & Agr, Guangdong Prov Key Lab Utilizat & Conservat Food &, Shaoguan 512005, Guangdong, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Inst Sci & Tech Informat, Natl Key Lab Trop Crop Breeding,Sanya Res Inst, Haikou 517101, Peoples R China

3.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Key Lab Mol Biophys, Chinese Minist Educ,Genet Engn Int Cooperat Base,C, Wuhan 430074, Peoples R China

4.Chinese Acad Trop Agr Sci, Hainan Inst Trop Agr Resources, Hainan Key Lab Protect & Utilizat Trop Bioresource, Haikou 517101, Peoples R China

5.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Peoples R China

6.Shaanxi Univ Technol, Coll Biol Sci & Engn, Shaanxi Prov Bioresource Key Lab, Hanzhong 723000, Peoples R China

关键词： Cassava; Abscisic acid; Abiotic stress; Drought; PP2C

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：6.5； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2024 年 206 卷

页码：

收录情况： SCI

摘要： Abscisic acid (ABA) signaling plays a crucial role in plant development and response to abiotic/biotic stress. However, the function and regulation of protein phosphatase 2C (PP2C), a key component of abscisic acid signaling, under abiotic stress are still unknown in cassava, a drought-tolerant crop. In this study, a cassava PP2C gene (MePP2C24) was cloned and characterized. The MePP2C24 transcripts increased in response to mannitol, NaCl, and ABA. Overexpression of MePP2C24 in Arabidopsis resulted in increased sensitivity to drought stress and decreased sensitivity to exogenous ABA. This was demonstrated by transgenic lines having higher levels of malondialdehyde (MDA), ion leakage (IL), and reactive oxygen species (ROS), lower activities of catalase (CAT) and peroxidase (POD), and lower proline content than wild type (WT) under drought stress. Moreover, MePP2C24 overexpression caused decrease in expression of drought-responsive genes related to ABA signaling pathway. In addition, MePP2C24 was localized in the cell nucleus and showed self-activation. Furthermore, many MePYLs (MePYL1, MePYL4, MePYL7-9, and MePYL11-13) could interact with MePP2C24 in the presence of ABA, and MePYL1 interacted with MePP2C24 in both the presence and absence of ABA. Additionally, MebZIP11 interacted with the promoter of MePP2C24 and exerted a suppressive effect. Taken together, our results suggest that MePP2C24 acts as a negative regulator of drought tolerance and ABA response.

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