文献类型： 外文期刊

作者： Zhang, Qian ¹ ; Li, Xing ¹ ; Geng-Li, Jiahong ¹ ; Yang, Jinyong ¹ ; Liu, Jingyi ¹ ; Wang, Ke ⁴ ; Cheng, Yuancan ¹ ; Liu, Jianping ¹ ; Xu, Feiyun ¹ ; Wang, Zhengrui ¹ ; Zhang, Kang ³ ; Zhang, Jianhua ⁵ ; Cheng, Feng ³ ; Xu, Weifeng ¹ ; Yuan, Wei ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Resources & Environm, Joint Int Res Lab Water & Nutrient Crop, Fujian Prov Key Lab Plant Funct Biol, Fuzhou, Peoples R China

2.Fujian Agr & Forestry Univ, Coll JunCao Sci & Ecol, Fuzhou, Peoples R China

3.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Hort Crops, State Key Lab Vegetable Biobreeding,Sino Dutch Joi, Inst Vegetables & Flowers,Minist Agr Rural Affairs, Beijing 100081, Peoples R China

4.Fujian Acad Agr Sci, Inst Resources Environm & Soil Fertilizer, Fuzhou, Peoples R China

5.Hong Kong Baptist Univ, Dept Biol, Hong Kong, Peoples R China

6.Chinese Univ Hong Kong, State Kay Lab Agrobiotechnol, Hong Kong, Peoples R China

关键词： cluster root formation; DNA methylation; melatonin synthesis; phosphorus deficiency; white lupin

期刊名称：PLANT CELL AND ENVIRONMENT （ 影响因子：6.3； 五年影响因子：7.7 ）

ISSN： 0140-7791

年卷期： 2025 年

页码：

收录情况： SCI

摘要： White lupin exhibits remarkable adaptability to phosphorus (P)-deficient soil through the development of cluster roots (CR), thereby enhancing P use sufficiency. Despite its crucial role, the underlying mechanism governing CR formation remains elusive. Here, we reveal an elevated DNA methylation level through whole-genome bisulfite sequencing in CR in response to P deficiency, particularly in gene and flanking regions, suggesting a responsive epigenetic mechanism. To further investigate the potential involvement of epigenetic remodelling, we treated lupin plants with the DNA methyltransferase (DNMT) inhibitor 5-azacytidine, which led to a disruption of total DNMT activity and impaired CR formation under phosphorus-deficient conditions. Integrated analysis of methylome and RNA-Seq highlights the methylation of CAFFEIC ACID O-METHYLTRANSFERASE 1 (COMT1), a key enzyme in melatonin synthesis, as pivotal for promoting CR formation in white lupin. Functional validation through overexpression or gene silencing of LaCOMT1 in transgenic lupin roots confirms the positive impact of LaCOMT1 on CR formation. Furthermore, melatonin application directly increases CR numbers, indicating the role of methylation-activated LaCOMT1 in promoting CR formation via melatonin synthesis. Those findings provide insights into the epigenomic landscape of white lupin, establishing a direct genetic link between epigenetic mechanisms and P-deficiency-induced CR formation.