Enzyme-modulated photothermal immunoassay of chloramphenicol residues in milk and egg using a self-calibrated thermal imager
文献类型： 外文期刊
作者： Wei, Luyu 1 ; Jiao, Fu 3 ; Wang, Zhilong 1 ; Wu, Long 4 ; Dong, Daming 3 ; Chen, Yiping 1 ;
作者机构： 1.Huazhong Agr Univ, Key Lab Environm Correlat Dietol, Minist Educ, Wuhan 430070, Peoples R China
2.Huazhong Agr Univ, Hubei HongShan Lab, Wuhan 430070, Peoples R China
3.Beijing Acad Agr & Forestry Sci, Natl Engn Res Ctr Intelligent Equipment Agr, Beijing 100097, Peoples R China
4.Hainan Inst Food Control, Key Lab Trop Fruits & Vegetables Qual & Safety Sta, Haikou 570314, Peoples R China
关键词： Chloramphenicol; Self-calibrated thermal imaging system (SCTIS); Oxidized 3; 3?; 5? -tetramethylbenzidine; (TMBox); Photothermal conversion; Immunoassay
期刊名称：FOOD CHEMISTRY （ 影响因子：9.231； 五年影响因子：8.795 ）
ISSN： 0308-8146
年卷期： 2022 年 392 卷
页码：
收录情况： SCI
摘要： Highly sensitive and accurate detection of chloramphenicol is of paramount importance for food safety. Herein, an enzyme-modulated photothermal immunosensor that uses a self-calibrated thermal imaging system (SCTIS) as signal read-out was developed for detecting chloramphenicol. In this immunosensor, alkaline phosphatase was used as a modulator of the photothermal conversion. It could hydrolyze the substrate into ascorbic acid, thereby reducing oxidized 3,3 ',5,5 '-tetramethylbenzidine, which exhibited a near-infrared laser-driven photothermal effect. For precise temperature measurement, the SCTIS was designed by using the temperature compensation of a ceramic chip to enable real-time self-calibration of the temperature. This SCTIS-based immunosensor could detect chloramphenicol with a LOD of 9 pg/mL in 2 h, and relative standard derivations from 3.95% to 13.58%. The average recoveries in milk and egg samples ranged from 76% to 114%. This versatile sensing strategy can detect various targets by altering recognition elements, thus has wide applicability in food safety testing and monitoring.
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