文献类型： 外文期刊

作者： Shah, Anish S. ¹ ; Hsu, Pei-Chun ¹ ; Chisholm, Chris ¹ ; Podolyan, Andriy ¹ ; Cameron, Keith ¹ ; Luo, Jiafa ² ; Stenger, Roland ³ ; Carrick, Sam ⁴ ; Hu, Wei ⁵ ; Ferguson, Scott A. ⁶ ; Wei, Wenhua ⁷ ; Shen, Jupei ⁸ ; Zhang, Limei ⁹ ; Liu, Hongbin ¹⁰ ; Zhao, Tongke ¹¹ ; Wei, Wenxue ¹² ; Ding, Weixin ¹³ ; Pan, Hong ¹⁴ ; Liu, Yimeng ¹⁵ ; Li, Bowen ¹⁶ ; Du, Jianjun ¹⁷ ; Di, Hong J. ¹ ;

作者机构： 1.Lincoln Univ, Ctr Soil & Environm Res, Lincoln, New Zealand

2.AgResearch, Hamilton, New Zealand

3.Lincoln Agritech, Ruakura Res Ctr, Hamilton, New Zealand

4.Manaaki Whenua Landcare Res, Lincoln, New Zealand

5.New Zealand Inst Plant & Food Res, Lincoln, New Zealand

6.Univ Otago, Dept Microbiol, Dunedin, New Zealand

7.Univ Otago, Dept Biochem, Dunedin, New Zealand

8.Fujian Normal Univ, Fuzhou, Peoples R China

9.Chinese Acad Sci, Res Ctr Ecoenvironm Sci, Beijing, Peoples R China

10.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Beijing, Peoples R China

11.Beijing Acad Agr & Forestry Sci, Beijing, Peoples R China

12.Chinese Acad Sci, Inst Subtrop Agr Ecol, Changsha, Peoples R China

13.Chinese Acad Sci, Inst Soil Sci, Nanjing, Peoples R China

14.Shandong Agr Univ, Coll Nat Resources & Environm, Tai An, Peoples R China

15.Beijing Normal Univ, Ctr Innovat & Dev, Zhuhai, Peoples R China

16.Hebei Agr Univ, Coll Nat Resources & Environm, Baoding, Peoples R China

17.Zhongkai Univ Agr & Engn, Coll Resources & Environm, Guangzhou, Peoples R China

18.Murdoch Univ, Food Futures Inst, Ctr Crop & Food Innovat, Sch Agr Sci, Perth, WA, Australia

关键词： comammox Nitrospira; ammonia oxidising archaea (AOA); ammonia oxidising bacteria (AOB); nitrification inhibitors; qPCR (quantitative PCR)

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.2； 五年影响因子：6.2 ）

ISSN：

年卷期： 2024 年 15 卷

页码：

收录情况： SCI

摘要： Introduction: Through the combined use of two nitrification inhibitors, Dicyandiamide (DCD) and chlorate with nitrogen amendment, this study aimed to investigate the contribution of comammox Nitrospira clade B, ammonia oxidizing bacteria (AOB) and archaea (AOA) to nitrification in a high fertility grassland soil, in a 90-day incubation study. Methods: The soil was treated with nitrogen (N) at three levels: 0 mg-N kg(-1) soil, 50 mg-N kg(-1) soil, and 700 mg-N kg(-1) soil, with or without the two nitrification inhibitors. The abundance of comammox Nitrospira, AOA, AOB, and nitrite oxidising bacteria (NOB) was measured using qPCR. The comammox Nitrospira community structure was assessed using Illumina sequencing. Results and Discussion: The results showed that the application of chlorate inhibited the oxidation of both NH4+ and NO2- in all three nitrogen treatments. The application of chlorate significantly reduced the abundance of comammox Nitrospira amoA and nxrB genes across the 90-day experimental period. Chlorate also had a significant effect on the beta diversity (Bray-Curtis dissimilarity) of the comammox Nitrospira clade B community. Whilst AOB grew in response to the N substrate additions and were inhibited by both inhibitors, AOA showed litle or no response to either the N substrate or inhibitor treatments. In contrast, comammox Nitrospira clade B were inhibited by the high ammonium concentrations released from the urine substrates. These results demonstrate the differential and niche responses of the three ammonia oxidising communities to N substrate additions and nitrification inhibitor treatments. Further research is needed to investigate the specificity of the two inhibitors on the different ammonia oxidising communities.