文献类型： 外文期刊

作者： Wang, Dongzhi ¹ ; Li, Yongpeng ² ; Wang, Haojie ¹ ; Xu, Yongxin ¹ ; Yang, Yiman ¹ ; Zhou, Yuxin ¹ ; Chen, Zhongxu ⁵ ; Zhou, Yuqing ¹ ; Gui, Lixuan ⁵ ; Guo, Yi ¹ ; Zhou, Chunjiang ² ; Tang, Wenqiang ² ; Zheng, Shuzhi ² ; Wang, Lei ¹ ; Guo, Xiulin ⁸ ; Zhang, Yingjun ⁹ ; Cui, Fa ¹⁰ ; Lin, Xuelei ¹ ; Jiao, Yuling ⁶ ; He, Yuehui ⁷ ; Li, Junming ² ; He, Fei ¹ ; Liu, Xigang ² ; Xiao, Jun ¹ ;

作者机构： 1.Chinese Acad Sci, Inst Genet & Dev Biol, Key Lab Plant Cell & Chromosome Engn, Beijing 100101, Peoples R China

2.Hebei Normal Univ, Coll Life Sci, Hebei Collaborat Innovat Ctr Cell Signaling, Hebei Key Lab Mol & Cellular Biol,Key Lab Mol & Ce, Shijiazhuang 050024, Peoples R China

3.Chinese Acad Sci, Inst Genet & Dev Biol, Ctr Agr Resources Res, Shijiazhuang 050022, Peoples R China

4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

5.Tcuni Inc, Dept Life Sci, Chengdu 610000, Peoples R China

6.Peking Univ, Ctr Quantitat Biol, Sch Life Sci, State Key Lab Prot & Plant Gene Res,Peking Tsinghu, Beijing 100871, Peoples R China

7.Peking Univ, Peking Tsinghua Ctr Life Sci, Sch Adv Agr Sci, State Key Lab Prot & Plant Gene Res, Beijing 100871, Peoples R China

8.Hebei Acad Agr & Forestry Sci, Inst Biotechnol & Food Sci, Plant Genet Engn Ctr Hebei Prov, Shijiazhuang 050051, Peoples R China

9.Hebei Acad Agr & Forestry Sci, Lab Crop Genet & Breeding Hebei, Inst Cereal & Oil Crops, Shijiazhuang 050035, Peoples R China

10.Ludong Univ, Coll Agr, Key Lab Mol Module Based Breeding High Yield & Abi, Yantai 264025, Peoples R China

11.JIC CAS, Ctr Excellence Plant & Microbial Sci CEPAMS, Beijing 100101, Peoples R China

关键词： wheat; exome capture sequencing; EMS mutagenesis; functional genomics

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2023 年 4 卷 4 期

页码：

收录情况： SCI

摘要： A better understanding of wheat functional genomics can improve targeted breeding for better agronomic traits and environmental adaptation. However, the lack of gene-indexed mutants and the low transforma-tion efficiency of wheat limit in-depth gene functional studies and genetic manipulation for breeding. In this study, we created a library for KN9204, a popular wheat variety in northern China, with a reference genome, transcriptome, and epigenome of different tissues, using ethyl methyl sulfonate (EMS) mutagenesis. This library contains a vast developmental diversity of critical tissues and transition stages. Exome capture sequencing of 2090 mutant lines using KN9204 genome-designed probes revealed that 98.79% of coding genes had mutations, and each line had an average of 1383 EMS-type SNPs. We identified new allelic var-iations for crucial agronomic trait-related genes such as Rht-D1, Q, TaTB1, and WFZP. We tested 100 lines with severe mutations in 80 NAC transcription factors (TFs) under drought and salinity stress and identified 13 lines with altered sensitivity. Further analysis of three lines using transcriptome and chromatin accessi-bility data revealed hundreds of direct NAC targets with altered transcription patterns under salt or drought stress, including SNAC1, DREB2B, CML16, and ZFP182, factors known to respond to abiotic stress. Thus, we have generated and indexed a KN9204 EMS mutant library that can facilitate functional genomics research and offer resources for genetic manipulation of wheat.