您好，欢迎访问中国热带农业科学院 机构知识库！
中国热带农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

The effect of extraction solution pH level on the physicochemical properties and alpha-glucosidase inhibitory potential of Fucus vesiculosus polysaccharide

文献类型： 外文期刊

作者： Zheng, Qianwen 1 ; Jia, Rui-Bo 1 ; Luo, Donghui 1 ; Lin, Lianzhu 1 ; Chen, Chong 4 ; Zhao, Mouming 1 ;

作者机构： 1.Chem & Chem Engn Guangdong Lab, Chaozhou Branch, Chaozhou 521000, Peoples R China

2.South China Univ Technol, Sch Food Sci & Engn, Guangzhou 510640, Peoples R China

3.Guangdong Ocean Univ, Sch Food Sci & Engn, Yangjiang 529500, Peoples R China

4.Chinese Acad Trop Agr Sci, Agr Prod Proc Res Inst, Hainan Key Lab Storage & Proc Fruits & Vegetables, Zhanjiang 524001, Guangdong, Peoples R China

关键词： Fucus vesiculosus polysaccharides; Extraction solution pH level; alpha-Glucosidase; Inhibitory mechanism

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）

ISSN： 0023-6438

年卷期： 2022 年 169 卷

页码：

收录情况： SCI

摘要： In this study, five Fucus vesiculosus polysaccharides (FVPs; FVP-2, FVP-4, FVP-7, FVP-10, and FVP-13) were extracted by different pH, and their physicochemical characteristics and alpha-glucosidase inhibitory activity were investigated. Results showed that extraction pH significantly affected the chemical composition, molecular weight, zeta potential, structure characterization, and rheological property of FVPs. The viscosity consistency index of Fucus vesiculosus polysaccharides at high concentration (40 mg/mL) were ranked in decreasing order as FVP-10 > FVP-4 > FVP-7 > FVP-2 > FVP-13, which was strictly in accordance with the order of Mw. Among the five polysaccharides, FVP-13 showed the strongest alpha-glucosidase inhibitory activity, which might be related to its the lowest Mw and relatively higher xylose content. Fluorescence quenching analysis indicated that the interaction of FVPs and alpha-glucosidase was predominated by a dynamic mechanism. Thermodynamic analysis showed that the main driving forces for the interaction was hydrophobic interaction. Circular dichroism spectrum analysis found that FVPs could induce the secondary structure of alpha-glucosidase changes like the decrease of beta-sheet content and the increase of beta-turn and random coil content to inhibit alpha-glucosidase activity. These results suggested that extraction with high pH was an efficient method to obtain FVPs with strong alpha-glucosidase inhibition activity.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国热带农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台