Anemarrhena asphodeloides Bunge-Fritillaria Cirrhosae Bulbus Herb Pair Alleviates Ovalbumin-Induced Asthma by Regulating Arachidonic Acid Pathway
文献类型： 外文期刊
作者： Tian, Xi 1 ; Cui, Qingfei 3 ; Wei, Jinhuan 1 ; Zhang, Qian 1 ; Zhang, Huiyi 1 ; Yang, Mengxin 1 ; Xing, Yiqi 4 ; Niu, Yukun 1 ; Li, Wenyu 1 ; Wang, Nan 1 ; Jin, Yiran 5 ; Du, Yingfeng 1 ;
作者机构： 1.Hebei Med Univ, Sch Pharm, Dept Pharmaceut Anal, Shijiazhuang, Hebei, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Cash Crop, Shijiazhuang, Hebei, Peoples R China
3.Shijiazhuang Yiling Pharmaceut Co Ltd, Shijiazhuang Biomed Academician Workstn, Shijiazhuang, Hebei, Peoples R China
4.Hebei Vocat Univ Ind & Technol, Architectural Engn Inst, Shijiazhuang, Hebei, Peoples R China
5.Hebei Med Univ, Hosp 2, Dept Pharm, Shijiazhuang, Hebei, Peoples R China
关键词： Anemarrhena asphodeloides Bunge-Fritillaria Cirrhosae Bulbus; anti-asthma; combination mechanisms; network pharmacology; metabolomics; RT-qPCR
期刊名称：DRUG DESIGN DEVELOPMENT AND THERAPY （ 影响因子：5.1； 五年影响因子：5.2 ）
ISSN： 1177-8881
年卷期： 2025 年 19 卷
页码：
收录情况： SCI
摘要： Background: In traditional Chinese Medicine (TCM), Zhimu (Anemarrhena asphodeloides Bunge, ZM)-Chuanbeimu (Fritillaria Cirrhosae Bulbus, CBM) (ZC) is one of the most classical herb pairs used in the treatment of lung diseases such as asthma. This study aimed to investigate how ZC affects asthma and its mechanism. Methods: Asthma model rats were sensitized by ovalbumin. The anti-asthma efficacy of ZM, CBM and ZC were evaluated through analysis of lung function, pathological sections and biochemical indices. Metabolomics based on UHPLC-QTOF-MS was conducted to determine the synergistic anti-asthma effect of combination therapy. Asthma targets and mechanism prediction were performed using network pharmacology. Then, the potential anti-asthma mechanism of ZC was explored using RT-qPCR. Results: According to the lung function test, Hematoxylin-Eosin Staining experiment, ZC herb pair had an obvious anti-asthma effect over either ZM or CBM alone. It has also been demonstrated that positive effect of ZC against Th1/Th2 immune imbalance. Both metabolomics and network pharmacology were highly enriched in the arachidonic acid metabolism pathway. The mRNA expression levels of ALOX5, PLA2G4A and CYP1A2, critical targets in arachidonic acid metabolism, were significantly down-regulated by RT-qPCR. Conclusion: By reducing the expression of cytokines and chemokines mediated by the arachidonic acid metabolism pathway, ZC could alleviate OVA-induced asthma in vivo. It was the first to demonstrate the complex mechanism of ZC for the treatment of asthma. Meanwhile, a new paradigm was established for evaluating the pharmacological effects of TCM drugs for asthma based on multiple mechanisms.
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