SpCTP3 from the hyperaccumulator Sedum plumbizincicola positively regulates cadmium tolerance by interacting with SpMDH1
文献类型： 外文期刊
作者： Li, Shaocui 1 ; He, Zhengquan 3 ; Qiu, Wenmin 1 ; Yu, Miao 1 ; Wu, Longhua 4 ; Han, Xiaojiao 1 ; Zhuo, Renying 1 ;
作者机构： 1.Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding Zhejiang Prov, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China
2.Zhejiang Acad Agr Sci, Zhejiang Xiaoshan Inst Cotton & Bast Fiber Crops, Zhejiang Inst Landscape Plants & Flowers, Hangzhou 311251, Peoples R China
3.China Three Gorges Univ, Biotechnol Res Ctr, Key Lab Three Gorges Reg Plant Genet & Germplasm E, Yichang 443002, Hubei, Peoples R China
4.Chinese Acad Sci, Inst Soil Sci, Key Lab Soil Environm & Pollut Remediat, Nanjing 210008, Peoples R China
关键词： SpCTP3; Cadmium; SpMDH1; Malic acid; Phytoremediation
期刊名称：JOURNAL OF HAZARDOUS MATERIALS （ 影响因子：13.6； 五年影响因子：12.7 ）
ISSN： 0304-3894
年卷期： 2024 年 472 卷
页码：
收录情况： SCI
摘要： Cadmium (Cd) is a heavy metal pollutant mainly originating from the discharge of industrial sewage, irrigation with contaminated water, and the use of fertilizers. The phytoremediation of Cd polluted soil depends on the identification of the associated genes in hyperaccumulators. Here, a novel Cd tolerance gene ( SpCTP3 ) was identified in hyperaccumulator Sedum plumbizincicola . The results of Cd 2 + binding and thermodynamic analyses, revealed the CXXC motif in SpCTP3 functions is a Cd 2 + binding site. A mutated CXXC motif decreased binding to Cd by 59.93%. The subcellular localization analysis suggested that SpCTP3 is primarily a cytoplasmic protein. Additionally, the SpCTP3 -overexpressing (OE) plants were more tolerant to Cd and accumulated more Cd than wild -type Sedum alfredii (NHE-WT). The Cd concentrations in the cytoplasm of root and leaf cells were significantly higher (53.75% and 71.87%, respectively) in SpCTP3 -OE plants than in NHE-WT. Furthermore, malic acid levels increased and decreased in SpCTP3 -OE and SpCTP3 -RNAi plants, respectively. Moreover, SpCTP3 interacted with malate dehydrogenase 1 (MDH1). Thus, SpCTP3 helps regulate the subcellular distribution of Cd and increases Cd accumulation when it is overexpressed in plants, ultimately Cd tolerance through its interaction with SpMDH1. This study provides new insights relevant to improving the Cd uptake by Sedum plumbizincicola
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