Agrobacterium tumefaciens-mediated transformation of embryogenic callus and CRISPR/Cas9-mediated genome editing in 'Feizixiao' litchi
文献类型： 外文期刊
作者： Wang, Shujun 1 ; Wang, Guo 1 ; Li, Huanling 1 ; Li, Fang 1 ; Wang, Jiabao 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China
2.Natl Key Lab Trop Crop Breeding, Haikou 571101, Hainan, Peoples R China
关键词： Litchi; Litchi chinensis Sonn.; Genetic transformation; Gene editing; Polyphenol oxidase (PPO)
期刊名称：HORTICULTURAL PLANT JOURNAL （ 影响因子：5.7； 五年影响因子：5.4 ）
ISSN： 2095-9885
年卷期： 2023 年 9 卷 5 期
页码：
收录情况： SCI
摘要： Litchi (Litchi chinensis Sonn.) is a type of commercially prevalent subtropical and tropical fruit. Since litchi has a highly heterozygous genetic background and a long reproductive cycle, conventional breeding methods (such as hybridization) have limited ability to nurture new litchi cultivars. Here, an efficient and stable Agrobacterium tumefaciens-mediated genetic transformation of embryogenic callus was established in 'Feizixiao' litchi. Transgenic materials were verified using polymerase chain reaction (PCR) analysis, b-glucuronidase (GUS) assay, and green fluorescent protein (GFP) assay. To implement the technology of the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/ associated protein 9 (CRISPR/Cas9) technology in 'Feizixiao' litchi and verify the validity of these transformation systems, the litchi polyphenol oxidase gene (LcPPO, JF926153) was knocked out. Various categories of mutations, covering base insertions, deletions, and substitutions, were found in transgenic materials via sequence analysis. The transformation system achieved high feasibility and efficiency, and the system of CRISPR/Cas9 was successfully employed to edit genes in 'Feizixiao' litchi. This work provides an essential foundation for investigating the functions of genes and accelerating litchi genetic improvement.
- 相关文献
作者其他论文 更多>>
-
Colletotrichum species associated with durian ( Durio zibethinus Murray) in Hainan, China
作者：Xu, Huan;Zhang, Zheyu;Xu, Huan;Zhang, Zheyu;Wang, Jiabao;Cao, Xueren;Zhou, Zhaoxi;West, Jonathan S.
关键词：Colletotrichum species; Durian; Identification; Phylogenetic; Pathogenicity
-
Pathogen-activated Chaetomium globosum G3 enhances iron competition and other antagonistic mechanisms to suppress maize seedling blight causal agent Fusarium verticillioides
作者：Ren, Chunlin;Li, Shiying;Li, Peipei;Wang, Yi;Li, Hui;Li, Fang;Han, Yanlai;Wang, Yi;Han, Yanlai;Yuan, Hongxia;Zhao, Qingyun
关键词：Chaetomium globosum; Biocontrol; Fusarium verticillioides; Iron competition; Fumonisin
-
Polyphenol oxidase gene editing changed the flavonoid composition and browning process of litchi ( Litchi chinensis Sonn.) callus
作者：Wang, Shujun;Li, Fang;Wang, Guo;Li, Huanling;Li, Xiaoxu;Cao, Xueren;Wang, Jiabao
关键词：Callus; Polyphenol oxidase; Flavonoids; Browning; Gene editing
-
Screening of highly efficient genotypes for in vitro regeneration in Litchi chinensis Sonn
作者：Wang, Guo;Li, Huanling;Wang, Jiabao;Liu, Yaoting;Zhang, Lei;Li, Zhiying
关键词：Litchi; Anthers; Callus; Somatic embryogenesis
-
The resistance of litchi fruit to Peronophythora litchii is associated with lignin biosynthesis and reactive oxygen species metabolism
作者：Li, Xiaoxu;Li, Fang;Li, Huanling;Wang, Guo;Wang, Shujun;Cao, Xueren;Sun, Jinhua;Cao, Lulu;Wang, Jiabao;Wang, Pengfei;Zhang, Lei;Wang, Jiabao
关键词：Litchi downy blight; Resistance mechanism; Lignin accumulation; ROS scavenging
-
Elucidating the mechanism of resistance to anthracnose in litchi leaves through transcriptome analysis
作者：Li, Fang;Wu, Ji;Lin, Qiying;Cao, Xueren;Li, Huanling;Wang, Shujun;Wang, Guo;Li, Xiaoxu;Wang, Jiabao;Wu, Ji;Zhang, Lei
关键词：
Litchi chinensis Sonn.; Anthracnose disease; Resistance response; Differentially expessed genes -
QTL mapping for branch-and leaf-related traits with a high-density SNP genetic map in litchi (Litchi chinensis Sonn.)
作者：Hu, Wei;Zhang, Lei;Li, Yao;Wang, Jiabao;Li, Fang;Li, Huanling;Cai, Rupeng;Lin, Qiying;Qin, Xiaoyun
关键词：Litchi; Branch-and leaf-related traits; High-density genetic map; Pleiotropic QTLs
