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Development of a highly sensitive aptamer-based electrochemical sensor for detecting saxitoxin based on K3Fe(CN)6 regulated silver nanoparticles

文献类型： 外文期刊

作者： Zeng, Wei 1 ; Tang, Xuemei 1 ; Wu, Ting 1 ; Han, Bingjun 2 ; Wu, Long 1 ;

作者机构： 1.Hainan Univ, Sch Food Sci & Engn, Key Lab Trop Fruits & Vegetables Qual & Safety Sta, Haikou 570228, Peoples R China

2.Chinese Acad Trop Agr Sci, Anal & Test Ctr, Hainan Prov Key Lab Qual & Safety Trop Fruits & Ve, Key Lab Qual & Safety Control Subtrop Fruits & Veg, Haikou 571101, Peoples R China

关键词： Marine biotoxin; Etching reaction; Aptasensor; Ratiometric detection; Food safety

期刊名称：ANALYTICA CHIMICA ACTA （ 影响因子：6.2； 五年影响因子：5.9 ）

ISSN： 0003-2670

年卷期： 2024 年 1287 卷

页码：

收录情况： SCI

摘要： Background: Saxitoxin (STX) is the most toxic marine toxin, which can pose several adverse effects on human health. High sensitivity, fast response, and low-cost detection of STX contamination are of significance to reducing the fishery and seafood industries' loss. Among the various types of biosensors, the electrochemical biosensors have been extensively studied in the detection of STX, but the electrode surface modification material is easy to fall off, resulting in unstable electrochemical signals and poor reproducibility. It is imperative to have a ratiometric electrochemical biosensor for STX.Results: In this study, we developed a novel aptamer-based electrochemical sensor (AECs) for the sensitive detection of STX based on a K3Fe(CN)6 regulated silver nanoparticles (Ag NPs) modified with aptamer. The AECs was constructed by immobilizing aptamer on Ag NPs surfaces. Under optimized conditions, the AECs showed a linear response towards STX in the range from 0.04 to 0.15 mu M with the regression equation of Y =-8.0 + 233.7 X (R2 = 0.9956). The limit of detection (LOD) was calculated to be 1 nM (based on 3 N/S), which is significantly lower than the regulatory limits for STX in seafood. Moreover, the AECs showed excellent sensitivity, reproducibility and stability, as well as the detection in samples with acceptable recovery ranged from 71.2 % to 93.8 %, demonstrating its broad application prospects in detection of STX in seafood samples.Significance: This work proposed an AECs to achieve sensitive detection of STX. A reaction system of K3Fe(CN)6 etched Ag NPs was introduced and used as the signal source to avoid the instability of the electrochemical signal, which can produce a ratiometric electrochemical signal output mode, improving the stability and sensitivity of electrochemical detection of STX.

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