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Characterization of HSP70 expression in drones of Apis cerana (Hymenoptera: Apidae)

文献类型： 外文期刊

作者： Wang, Deqian 1 ; Dong, Jie 1 ; Huang, Minjie 1 ; Ding, Guiling 2 ; Huang, Jiaxing 2 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Peoples R China

2.Chinese Acad Agr Sci, Inst Apicultural Res, Key Lab Pollinating Insect Biol, Minist Agr & Rural Affairs, Beijing 100093, Peoples R China

关键词： Honeybee; Apis cerana; Heat shock proteins; HSP70; Gene expression

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.58； 五年影响因子：1.575 ）

ISSN： 1226-8615

年卷期： 2022 年 25 卷 1 期

页码：

收录情况： SCI

摘要： Heat shock proteins (HSPs) constitute a superfamily of molecular chaperones that are rapidly biosynthesized in response to various biotic and abiotic factors. In this study, we first cloned the full-length HSP70 gene of the Eastern honeybee Apis cerana. Then, using real-time quantitative PCR, we explored HSP70 expression profiles in drones at different developmental stages, ages, and reproductive statuses (with and without semen). The fulllength HSP70 cDNA is 2421 bp, including a 1953-bp open reading frame (ORF) that encodes a polypeptide of 650 amino acids. The HSP70 gene consists of one intron and two exons. The phylogenetic analysis revealed that the HSP70 genes of A. cerana and Apis mellifera are the most closely related. We observed HSP70 expression at all selected developmental stages and detected the highest expression in pupae with an unpigmented body cuticle and brown eyes (Pb) and much lower expression in larvae hatched within 72 h. In adult drones of different ages, the highest expression level of HSP70 was observed in 16-day-old drones; significantly lower accumulation of HSP70 mRNA was detected in 4-day-old drones. There was no significant difference in HSP70 expression between drones with and without semen captured at the entrance, while the HSP70 gene expression level strikingly differed between drones captured at the entrance and the drones collected within the hive. Our study suggests that HSP70 might play a critical role in drone development and during reproductive mating events.

  • 相关文献

[1]Characterization of HSP70 expression in drones of Apis cerana (Hymenoptera: Apidae). Wang, Deqian,Dong, Jie,Huang, Minjie,Ding, Guiling,Huang, Jiaxing. 2022

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