Exploring the molecular mechanism of OsROS1a in regulating resistance to bacterial leaf streak through transcriptome and DNA methylation profiling in rice (Oryza sativa L.)
文献类型： 外文期刊
作者： Xie, Xiaofang 1 ; Yang, Xuansong 2 ; Lu, Libin 3 ; Li, Tong 1 ; Qin, Mingyue 1 ; Guan, Huazhong 2 ; Zheng, Yan 1 ; Lan, Tao 2 ; Wu, Weiren 2 ;
作者机构： 1.Fujian Agr & Forestry Univ, Coll Life Sci, Fuzhou 350002, Peoples R China
2.Fujian Agr & Forestry Univ, Fujian Key Lab Crop Breeding Design, Fuzhou 350002, Peoples R China
3.Fujian Acad Agr Sci, Fuzhou 350003, Peoples R China
关键词：
期刊名称：BMC GENOMICS （ 影响因子：3.7； 五年影响因子：4.2 ）
ISSN： 1471-2164
年卷期： 2025 年 26 卷 1 期
页码：
收录情况： SCI
摘要： BackgroundDNA demethylases regulate the levels of genomic DNA methylation in plants. The demethylase REPRESSOR OF SILENCING 1 (ROS1) is a crucial factor for modulating gene expression in plant disease responses. Bacterial leaf streak (BLS), caused by Xanthomonas oryzae pv. Oryzicola (Xoc), is a highly destructive disease in rice. BLS resistance in rice is known to be quantitatively inherited, but the mechanisms by which DNA methylation controls BLS resistance remain poorly understood.ResultsIn this study, we knocked down OsROS1a expression in the rice variety Nipponbare using RNA interference (RNAi). The average BLS lesion length in the transgenic (T2) OsROS1a-RNAi (RS) lines was significantly reduced compared to that in wild-type Nipponbare plants (NP). Using whole-genome bisulfite sequencing (WGBS) and RNA-sequencing (RNA-seq), we analyzed the DNA methylations and transcriptomes of RS lines in comparison with NP at 0 (control), 5, 10, and 24 h post-inoculation with Xoc. A total of 1080 differentially expressed genes (DEGs) related to Xoc infection between the NP and RS lines were identified, which could be grouped into 8 clusters by K-means analysis. The DEGs in cluster 1 were enriched in the biological process related to defense response, response to stress, oxidation-reduction, etc. Integration of the methylome and transcriptome data revealed 112 overlapping differentially methylated and expressed genes (DMEGs). Gene Ontology (GO) analysis showed that the DMEGs were mainly involved in biological processes, such as metabolic process, cellular process, responses to stimulus, signaling, and immune system processes. KEGG pathway enrichment analysis revealed that these DMEGs were enriched in pathways related to glutathione metabolism, plant-pathogen interaction, cysteine and methionine, diterpenoid biosynthesis, photosynthesis, and starch and sucrose. Additionally, LOC_Os09g12660, encoding the glucose-1-phosphate adenylyl transferase large subunit, a chloroplast precursor involved in synthesizing activated glycosyl donor, showed strong potential to contribute to BLS resistance.ConclusionOsROS1a plays a crucial role in modulating rice resistance to bacterial leaf streak (BLS) caused by Xanthomonas oryzae pv. oryzicola (Xoc). These findings provide valuable insights into the role of OsROS1a in BLS resistance.
- 相关文献
作者其他论文 更多>>
-
Editing the RR-TZF Gene Subfamily in Rice Uncovers Potential Risks of CRISPR/Cas9 for Targeted Genetic Modification
作者：Zhou, Shufen;Lu, Xiaozhuan;Wu, Weiren;Zhou, Shufen;Tian, Dagang;Liu, Huaqing;Zhang, Di;Chen, Rui;Yang, Shaohua;Wang, Feng
关键词：CRISPR/Cas9;
RR-TZF gene subfamily; rice (Oryza sativa L.); off-target effects; inheritable mutagenicity -
Genome-wide identification and analysis of SmRR gene family in eggplant (Solanum melongena L.) and their response to abiotic stress and auxin
作者：Li, Wenjing;Li, Hui;Lin, Yanyu;Li, Yutong;Xie, Xiaofang;Wu, Weiren;Zhou, Yuanchang;Zheng, Yan;Li, Wenjing;Zhou, Yuanchang;Zheng, Xuefang;Li, Hui;Lin, Yanyu;Li, Yutong;Xie, Xiaofang;Zheng, Yan;Li, Wenjing;Li, Hui;Lin, Yanyu;Li, Yutong;Xie, Xiaofang;Wu, Weiren;Zhou, Yuanchang;Zheng, Yan
关键词：Eggplant; RR gene family; Genome-wide identification; Abiotic stress; IAA treatment
-
The MADS6, JAGGED, and YABBY proteins synergistically determine floral organ development in rice
作者：Cai, Zhengzheng;Li, Jieqiong;Su, Yuanyuan;Zheng, Lili;Zhang, Jianwei;Zhu, Miaomiao;Kong, Lan;Ye, Yanfang;Wu, Weiren;Duan, Yuanlin;Cai, Zhengzheng;Li, Jieqiong;Su, Yuanyuan;Zheng, Lili;Zhang, Jianwei;Zhu, Miaomiao;Kong, Lan;Ye, Yanfang;Wu, Weiren;Duan, Yuanlin;Qiu, Bingwen;Kong, Lan;Xue, Yongbiao;Xue, Yongbiao
关键词：
-
JAGGED and DROOPING LEAF synergistically establish sexual organs and terminate floral meristem in rice
作者：Cai, Zhengzheng;Su, Yuanyuan;Kong, Lan;Ren, Guangxin;Zheng, Lili;Zhu, Miaomiao;Ye, Yanfang;Wu, Weiren;Duan, Yuanlin;Cai, Zhengzheng;Su, Yuanyuan;Kong, Lan;Ren, Guangxin;Zheng, Lili;Zhu, Miaomiao;Ye, Yanfang;Wu, Weiren;Duan, Yuanlin;Kong, Lan;Qiu, Bingwen;Hong, Zonglie;Xue, Yongbiao;Xue, Yongbiao
关键词：
DROOPING LEAF ; floral meristem termination; floral organ specification;KNOTTED-LIKE HOMEOBOX ;JAGGED ;SUPERWOMAN1 ; rice -
Comprehensive molecular evolutionary analysis of small heat shock proteins in five diploid Gossypium species
作者：Fan, Kai;Qian, Zhengyi;He, Yuxi;Chen, Jiayuan;Ye, Fangting;Zhu, Xiaogang;Lin, Wenxiong;Lan, Tao;Li, Zhaowei;Fan, Kai;Qian, Zhengyi;He, Yuxi;Chen, Jiayuan;Ye, Fangting;Zhu, Xiaogang;Lin, Wenxiong;Li, Zhaowei;Fan, Kai;Qian, Zhengyi;He, Yuxi;Chen, Jiayuan;Ye, Fangting;Zhu, Xiaogang;Lin, Wenxiong;Lan, Tao;Li, Zhaowei;Fan, Kai;Qian, Zhengyi;He, Yuxi;Chen, Jiayuan;Ye, Fangting;Zhu, Xiaogang;Lin, Wenxiong;Li, Zhaowei;Cui, Lili
关键词：
-
Functional and evolutionary comparative analysis of the DIR gene family in Nicotiana tabacum L. and Solanum tuberosum L.
作者：Li, Tong;Chen, Rui;He, Huaqin;Wang, Ruiqi;Xie, Xiaofang;Luo, Wenbin;Lu, Libing;Du, Chaofan;Lin, Xiaolu;Lin, Guojian;Xie, Xiaofang
关键词：Dirigent (DIR); Biotic stress; Expression analysis; Phylogenetic analysis; Nicotiana tabacum L.; Solanum tuberosum L.
-
Effect of ADH7 gene loss on fusel oil metabolism of Saccharomyces cerevisiae for Huangjiu fermentation
作者：Liang, Zhangcheng;Lin, Xiaozi;Su, Hao;He, Zhigang;Li, Weixin;Zheng, Yan;Liang, Zhangcheng;He, Bing;Lin, Xiaozi;Su, Hao;He, Zhigang;Li, Weixin;Zheng, Yan;Chen, Jinxing;Liang, Zhangcheng;Lin, Xiaozi;He, Zhigang;Li, Weixin;He, Zhigang;Chen, Jinxing
关键词：Saccharomyces cerevisiae; ADH7 gene; Fusel oil production; Gene complementation; Gene function analysis
