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CPK28-mediated phosphorylation enhances nitrate transport activity of NRT2.1 during nitrogen deprivation

文献类型： 外文期刊

作者： Yue, Lindi 1 ; Liu, Mengyuan 2 ; Liao, Jiahui 1 ; Zhang, Kaina 1 ; Wu, Wei-Hua 1 ; Wang, Yang 1 ;

作者机构： 1.China Agr Univ, Coll Biol Sci, State Key Lab Plant Environm Resilience, Beijing 100193, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Beijing Key Lab Maize DNA Fingerprinting & Mol Bre, Beijing 100097, Peoples R China

关键词： CPK28; high-affinity nitrate uptake; nitrate transport; NRT2.1; protein phosphorylation

期刊名称：NEW PHYTOLOGIST （ 影响因子：8.1； 五年影响因子：10.3 ）

ISSN： 0028-646X

年卷期： 2025 年 245 卷 1 期

页码：

收录情况： SCI

摘要： Nitrate (NO3-) serves as the primary inorganic nitrogen source assimilated by most terrestrial plants. The acquisition of nitrate from the soil is facilitated by NITRATE TRANSPORTERS (NRTs), with NRT2.1 being the key high-affinity nitrate transporter. The activity of NRT2.1, which has multiple potential phosphorylation sites, is intricately regulated under various physiological conditions. Here, we discovered that CALCIUM-DEPENDENT PROTEIN KINASE 28 (CPK28) positively regulates nitrate uptake under nitrogen deprivation conditions. We found CPK28 as the kinase targeted by immunoprecipitation followed by mass spectrometry and examined the in-planta phosphorylation status of NRT2.1 in cpk28 mutant plants by employing quantitative MS-based phosphoproteomics. Through a combination of in vitro phosphorylation experiment and immunoblotting using phospho-specific antibody, we successfully demonstrated that CPK28 specifically phosphorylates NRT2.1 at Ser21. Functional analysis conducted in Xenopus oocytes revealed that co-expression of CPK28 significantly enhanced high-affinity nitrate uptake of NRT2.1. Further investigation using transgenic plants showed that the phosphomimic variant NRT2.1S21E, but not the nonphosphorylatable variant NRT2.1S21A, fully restored high-affinity 15NO3- uptake ability in both nrt2.1 and cpk28 mutant backgrounds. This study clarifies that the kinase activity of CPK28 is promoted during nitrogen deprivation conditions. These significant findings provide valuable insights into the intricate regulatory mechanisms that govern nitrate-demand adaptation.

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