Protective Effect of Resveratrol on Immortalized Duck Intestinal Epithelial Cells Exposed to H2O2
文献类型： 外文期刊
作者： Zhou, Ning 1 ; Tian, Yong 1 ; Wu, Hongzhi 3 ; Cao, Yongqing 1 ; Li, Ruiqing 1 ; Zou, Kang 2 ; Xu, Wenwu 1 ; Lu, Lizhi 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Sci & Vet, State Key Lab Managing Biot & Chem Threats Qual &, Hangzhou 310000, Peoples R China
2.Nanjing Agr Univ, Coll Anim Sci & Technol, Nanjing 210095, Peoples R China
3.Chinese Acad Trop Agr Sci, Trop Crop Genet Resource Res Inst, Haikou 571101, Hainan, Peoples R China
关键词： resveratrol; epithelial cells; duck; oxidative stress; apoptosis
期刊名称：MOLECULES （ 影响因子：4.927； 五年影响因子：5.11 ）
ISSN：
年卷期： 2022 年 27 卷 11 期
页码：
收录情况： SCI
摘要： Resveratrol is a polyphenolic compound with anti-oxidation effects. The mechanisms underlying the antioxidant effects of resveratrol in duck intestinal epithelial cells remain unclear. The protective effects of resveratrol against oxidative stress induced by H2O2 on immortalized duck intestinal epithelial cells (IDECs) were investigated. IDECs were established by transferring the lentivirus-mediated simian virus 40 large T (SV40T) gene into small intestinal epithelial cells derived from duck embryos. IDECs were morphologically indistinguishable from the primary intestinal epithelial cells. The marker protein cytokeratin 18 (CK18) was also detected in the cultured cells. We found that resveratrol significantly increased the cell viability and activity of catalase and decreased the level of intracellular reactive oxygen species and malondialdehyde, as well as the apoptosis rate induced by H2O2 (p < 0.05). Resveratrol up-regulated the expression of NRF2, p-NRF2, p-AKT, and p-P38 proteins and decreased the levels of cleaved caspase-3 and cleaved caspase-9 and the ratio of Bax to Bcl-2 in H2O2-induced IDECs (p < 0.05). Our findings revealed that resveratrol might alleviate oxidative stress by the PI3K/AKT and P38 MAPK signal pathways and inhibit apoptosis by altering the levels of cleaved caspase-3, cleaved caspase-9, Bax, and Bcl-2 in IDECs exposed to H2O2.
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