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Distribution characteristics and diversities of cbb and coxL genes in paddy soil profiles from southern China

文献类型： 外文期刊

作者： Peng, Wentao 1 ; Wang, Yan 1 ; Zhu, Xiuxiu 1 ; Xu, Liufeng 1 ; Zhao, Juan 1 ; Cui, Zhongli 1 ; Cao, Hui 1 ;

作者机构： 1.Nanjing Agr Univ, Coll Life Sci, Minist Agr & Rural Affairs, Key Lab Agr Environm Microbiol, Nanjing 210095, Peoples R China

2.Chinese Acad Trop Agr Sci, Rubber Res Inst, Haikou 571101, Hainan, Peoples R China

关键词： Calvin-Benson-Bassham cycle; carbon dioxide fixation; carbon monoxide dehydrogenase; ribulose-1,5-bisphosphate carboxylase/oxygenase

期刊名称：PEDOSPHERE （ 影响因子：3.911； 五年影响因子：4.814 ）

ISSN： 1002-0160

年卷期： 2021 年 31 卷 6 期

页码：

收录情况： SCI

摘要： The Calvin-Benson-Bassham cycle and Wood-Ljungdahl pathway are two well-known ones in the six carbon sequestration pathways, but the current knowledge of their occurrence in different layers of agricultural soil profiles is poor. In this study, the diversities of three genes encoding ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO), i.e., genes encoding the green-like (cbbLG) and red-like (cbbLR) forms of RubisCO I and encoding RubisCO II (cbbM), and the gene encoding carbon monoxide dehydrogenase large subunit (coxL) from five paddy soils in southern China were investigated by real-time quantitative polymerase chain reaction, restriction fragment length polymorphism (RFLP) analysis, and clone library. The abundances of the four genes ranged from 10(7) to 10(9) copies g(-1) soil, and the cbbLR gene outnumbered the other three genes in all soil samples, suggesting important roles they play in carbon dioxide (CO2) fixation. In addition, it was found that the copy numbers of cbbLR and cbbLG decreased with increasing soil depth, while the copy numbers of cbbM and coxL decreased in the shallow depths but increased with increasing soil depth. The results of RFLP showed a larger Shannon index (H) in the deeper soil layers among the four gene clone libraries, indicating that the community diversity in these soil layers was greater. The cbbLG gene had relatively low diversity (at genus level), and most of the sequences were classified as Sideroxydans and Thiobacillus. In contrast, the highly diverse groups were found in the other three gene clone libraries (cbbLR, cbbM, and coxL), most of which were distantly related to known sequences, even forming separate clusters. In summary, this study provides a new insight into CO2 fixers along agricultural soil profiles by comparing four bacterial genes.

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