文献类型： 外文期刊

作者： Xu, Xiao Ping ¹ ; Cao, Qing Ying ¹ ; Guan, Qing Xu ¹ ; Mohammadi, Mohammad Aqa ¹ ; Cai, Rou Di ¹ ; Chen, Xiao Hui ¹ ; Zhang, Zi Hao ¹ ; Chen, Yu Kun ¹ ; Xuhan, Xu ⁴ ; Lin, Yu Ling ¹ ; Lai, Zhong Xiong ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Inst Hort Biotechnol, Fuzhou 350002, Fujian, Peoples R China

2.Fujian Acad Agr Sci, Inst Biotechnol, Fuzhou 350003, Fujian, Peoples R China

3.Fujian Agr & Forestry Univ, Coll Hort, Fuzhou 350002, Fujian, Peoples R China

4.Inst Rech Interdisciplinaire Toulouse IRIT ARI, F-31300 Toulouse, France

关键词： MiRNA; Somatic embryogenesis; Cell wall; MiRNA-agomir; antagomir; Longan

期刊名称：PLANT SCIENCE （ 影响因子：5.363； 五年影响因子：5.454 ）

ISSN： 0168-9452

年卷期： 2022 年 323 卷

页码：

收录情况： SCI

摘要： The dynamic alterations in cell wall (CW) biosynthesis play an essential role in physiological isolation during the plant somatic embryogenesis (SE). However, the mechanisms underlying the functions of cell wall-associated miRNAs (CW-miRNA) remain poorly understood in plant SE. Here, we have identified 36 distinct candidate miRNAs associated with CW biosynthesis from longan third-generation genome as well as miRNA transcriptome, and modified RLM-RACE validated four distinct miRNA, which specifically targeted four CW-related genes. More importantly, we found that the dlo-miR397a-antagomir significantly enhanced DlLAC7 expression and improved laccase activity. Interestingly, inhibition of dlo-miR397a increased CW lignin deposition and promoted the tightening of protodermal cell by miRNA-mimic technology during early SE. Moreover, overexpression of dlo-miR408-3p (dlo-miR408-3p-agomir) markedly decreased DlLAC12 expression. dlo-miR408-3p-agomir activated rapid cell division, thus promoting the globular embryo (GE) development, which might be due to high DNA synthesis activity in protoepidermal cells, rather than affecting lignin synthesis. The subcellular location also indicated that both DlLAC7 and DlLAC12 proteins were primarily localized in CW and regulated CW biosyn-thesis. Overall, our findings provided new insight on the molecular regulatory networks comprising various miRNAs associated with cell wall, and established that dlo-miR397a and dlo-miR408-3p played differential roles during early SE in longan. The findings also shed some light on the potential role of miRNA target DlLAC regulating in vivo embryonic development of plant.