GmbZIP4a/b Positively Regulate Nodule Number by Affecting Cytokinin Biosynthesis in Glycine max
文献类型： 外文期刊
作者： Meng, Yongjie 1 ; Wang, Nan 2 ; Wang, Xin 1 ; Qiu, Zhimin 3 ; Kuang, Huaqin 1 ; Guan, Yuefeng 1 ;
作者机构： 1.Guangzhou Univ, Innovat Ctr Mol Genet & Evolut, Sch Life Sci, Guangdong Prov Key Lab Plant Adaptat & Mol Design, Guangzhou 510006, Peoples R China
2.Inner Mongolia Univ, Sch Life Sci, Hohhot 010021, Peoples R China
3.Zhejiang Acad Agr Sci, Zhejiang Inst Subtrop Crops, 334 Xueshan Rd, Wenzhou 325005, Peoples R China
关键词： basic leucine zipper 4; cytokinin; soybean; nodule
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）
ISSN： 1661-6596
年卷期： 2024 年 25 卷 24 期
页码：
收录情况： SCI
摘要： Legumes have the capability to form nodules that facilitate symbiotic nitrogen fixation (SNF) with rhizobia. Given the substantial energy consumption during the process of SNF, legumes need to optimize nodule number in response to everchanging environmental scenarios. The TGACG BINDING FACTOR1/4 (TGA1/4) are key players in the basal immune response of plants. In this study, both beta-glucuronidase staining and quantitative reverse transcription PCR (qRT-PCR) demonstrated that both GmbZIP4a and GmbZIP4b are inducible upon rhizobial inoculation. To investigate their roles further, we constructed gmbzip4a/b double mutants using CRISPR/Cas9 system. Nodulation assessments revealed that these double mutants displayed a reduction in the number of infection threads, which subsequently resulted in a decreased nodule number. However, the processes associated with nodule development including nodule fresh weight, structural characteristics, and nitrogenase activity, remained unaffected in the double mutants. Subsequent transcriptome analyses revealed that zeatin biosynthesis was downregulated in gmbzip4a/b mutants post rhizobial inoculation. Supporting these findings, genes associated with cytokinin (CTK) signaling pathway were upregulated in Williams 82 (Wm82), but this upregulation was not observed in the double mutants after rhizobial treatment. These results suggest that GmbZIP4a/b positively influences nodule formation by promoting the activation of the CTK signaling pathway during the early stages of nodule formation.
- 相关文献
作者其他论文 更多>>
-
Insect residual streams supplement improves chili pepper growth: Insights into the role of rhizosphere soil microbiome and metabolome
作者：Zhou, Hanghai;Zheng, Xusong;Shen, Qi;Yao, Xiaohong;Sun, Hong;Wang, Xin;Wu, Yifei;Tang, Jiangwu;Zhu, Zhigang;Yang, Chenghu;Li, Hua;Liu, Yuxue;Jiang, Lijia;Zhang, Chunfang
关键词：Insect farming; Insect residual stream; Chili pepper; Plant growth; Rhizosphere microbial community; Metabolic profile
-
Integrated Analysis of the Transcriptome and Metabolome Reveals the Network Regulating Fruit Taste in Sponge Gourd (Luffa cylindrica)
作者：Chai, Yaqian;Qiu, Wenjing;Gao, Luyao;Dong, Wenqi;Zhang, Peng;Zhou, Shengjun;Wang, Xin;Zhu, Yuqiang;Sun, Yuyan;Chai, Yaqian;Li, Zhikun
关键词：sponge gourd; taste; transcriptome; metabolome; mechanism
-
Enhancing Akkermansia growth via phytohormones: a strategy to modulate the gut-bone axis in postmenopausal osteoporosis therapy
作者：Zhao, Zhiqi;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Sun, Haibiao;Han, Xiaoqiang;Deng, Yixuan;Li, Li
关键词：Osteoporosis; Phytohormones; Soybean isoflavone; Naringin; Denosumab; Gut microbiome
-
Raffinose-metabolizing bacteria impair radiation-associated hematopoietic recovery via the bile acid/FXR/NF-κB signaling pathway
作者：Jiao, Yang;Ren, Jiawei;Yuan, Nan;Shen, Jiaqi;Guo, Hongjuan;Cao, Jianping;Wu, Depei;Jiao, Yang;Ren, Jiawei;Yuan, Nan;Guo, Hongjuan;Cao, Jianping;Jiao, Yang;Wu, Depei;Qi, Xiaofei;Xie, Shichang;Zhou, Zhemin;Shen, Jiaqi;Wu, Depei;Qi, Xiaofei;Shen, Jiaqi;Wu, Depei;Qi, Xiaofei;Shen, Jiaqi;Wu, Depei;Yin, Huafang;Wang, Jian;Wang, Xin;Wang, Xin;Zhou, Zhemin;Zhou, Zhemin
关键词：Radiation-induced hematopoietic injury; gut microbiome;
Bacteroides acidifaciens ; raffinose-metabolizing bacteria; Intestinal-Hematopoietic Axis; UDCA; FXR -
Soil heterogeneity influences the biotoxicity of photoaged tire wear particles in Eisenia fetida: A comparative assessment
作者：Zhou, Hanghai;Wang, Xin;Sun, Hong;Li, Hua;Yao, Xiaohong;Wang, Yuan;Jin, Junwei;Tang, Jiangwu;Wu, Zhou;Li, Yongfu;Zhou, Jianjiang;Jiang, Lijia;Zhang, Chunfang
关键词：Tire wear particle; Photoaging; Earthworm; Soil heterogeneity; Oxidative damage
-
Regulatory Roles of Quercetin in Alleviating Fructose-Induced Hepatic Steatosis: Targeting Gut Microbiota and Inflammatory Metabolites
作者：Li, Jinjun;Li, Xiaoqiong;Li, Jinjun;Li, Xiaoqiong;Li, Jinjun;Zhao, Zhiqi;Li, Xinxin;Zhu, Liying;Wang, Xin;Li, Xiaoqiong;Li, Jinjun;Deng, Yixuan;Li, Li
关键词：fructose; gut microbiota; hepatic steatosis; quercetin
-
6PPD-quinone exposure induces oxidative damage and physiological disruption in Eisenia fetida: An integrated analysis of phenotypes, multi-omics, and intestinal microbiota
作者：Zhou, Hanghai;Wang, Xin;Sun, Hong;Li, Hua;Wang, Yuan;Yao, Xiaohong;Tang, Jiangwu;Wu, Zhou;Wu, Zhou;Jiang, Lijia;Zhang, Chunfang;Yan, Zhongyong
关键词：Earthworm; 6PPD-quinone; Oxidative stress; Multi-omics; Intestinal microbial community
