文献类型： 外文期刊
作者： Chen, Zhao 1 ; Aman, Khan 1 ; Zhang, Wei 2 ; Wang, Jinghong 2 ; Zhang, Fangzheng 2 ; Shen, Guinan 2 ; Hong, Yanhua 2 ; Wei, Dan 3 ; Cui, Zongjun 4 ; Wang, Weidong 1 ;
作者机构： 1.Northeast Forestry Univ, Coll Life Sci, Key Lab Saline Alkali Vegetat Ecol Restorat, Minist Educ, Harbin 150040, Peoples R China
2.Heilongjiang Bayi Agr Univ, Coll Life Sci & Biotechnol, Key Lab Low Carbon Green Agr Northeastern China, Minist Agr & Rural Affairs PR China, Daqing 163319, Heilongjiang, Peoples R China
3.Beijing Acad Agr & Forestry, Inst Plant Nutr & Resource Environm, Beijing 100097, Peoples R China
4.China Agr Univ, Coll Agr, Beijing 100094, Peoples R China
关键词： Anaerobic digestion; Single substrate; Methane; Microbial diversity; Metabolic mechanism
期刊名称：BIORESOURCE TECHNOLOGY （ 影响因子：9.0； 五年影响因子：9.5 ）
ISSN： 0960-8524
年卷期： 2024 年 413 卷
页码：
收录情况： SCI
摘要： Simplified anaerobic digestion (SAD) of substrates facilitates microbial methanogenic pathways. In this study, a methane-producing microbial consortium from cow dung was enriched to determine the metabolism and metabolic pathway in the SAD of methanol. The results showed that methanol as a sole substrate produced 167 mL of methane at 10 days significantly higher than 58 mL, 17.6 mL, and 4 mL generated when methanol was combined with sodium formate, sodium formate alone, or sodium acetate. The relative abundance of Methanobacterium, Candidatus_Methanomethylophilus, Methanomassiliicoccus, and Methanosarcina was increased by 5.96 %, 3.77 %, 2.85 %, and 0.14 % in the methanol substrate of AD, respectively. Macrogenome sequencing indicates that methanol wasconverted into Methyl-CoM in the presence of Methanosarcina, , which combines with Coenzyme B to produce methane. This study revealed that methanol is converted into methane by a simple pathway.
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