文献类型： 外文期刊

作者： Xiao, Wei ¹ ; Lian, Zong-Qiang ² ; Wu, Jian-Ping ¹ ; Wu, Xu-Dong ¹ ; Yu, Zhao-Xi ² ; Sai, Qing-Yun ² ;

作者机构： 1.Gansu Agr Univ, Coll Anim Sci & Technol, 1 Yingmen Village, Lanzhou 730070, Gansu, Peoples R China

2.Ningxia Fisheries Res Inst, Yinchuan, Ningxia, Peoples R China

3.Gansu Acad Agr Sci, Lanzhou, Gansu, Peoples R China

关键词： Growth-related genes; Muscle tissue; RNA-sequencing; Silurus lanzhouensis

期刊名称：FISHERIES SCIENCE （ 影响因子：2.148； 五年影响因子：1.933 ）

ISSN： 0919-9268

年卷期： 2022 年 88 卷 5 期

页码：

收录情况： SCI

摘要： To obtain transcriptomic insights into the growth regulation traits of Silurus lanzhouensis, this study employed de novo RNA sequencing to analyze the muscle transcriptome of the three heaviest (SLA) and three lightest (SLB) individuals from the same S. lanzhouensis family. Notably, the body weight, total length, and head length of the two groups were found to be significantly different (unpaired t test, P < 0.05). A total of 163,949 transcripts and 89,579 genes were identified, of which 843 differentially expressed genes (DEGs) were identified between the two groups (366 up-regulated and 477 down-regulated in the SLA group). The results were further validated via quantitative real-time polymerase chain reaction of 16 selected DEGs and were deemed highly reliable. The differences in growth rates between the two groups were attributed to the DEGs associated with carbohydrate metabolism, cysteine and methionine metabolism, and cardiac muscle contraction. Hex-t2, Fbp2, and Pepck were the most promising candidate genes to explain the differences in growth rate between SLA and SLB. These results provide valuable insights into fish growth traits and may aid in the identification of biomarkers for the establishment of selection models for S. lanzhouensis and other cultured fish species.