Dual RNA-seq Reveals the Global Transcriptome Dynamics of Ralstonia solanacearum and Pepper (Capsicum annuum) Hypocotyls During Bacterial Wilt Pathogenesis
文献类型： 外文期刊
作者： Du, Heshan 1 ; Yang, Jingjing 1 ; Chen, Bin 1 ; Zhang, Xiaofen 1 ; Xu, Xiulan 1 ; Wen, Changlong 1 ; Geng, Sansheng 1 ;
作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Beijing 100097, Peoples R China
2.Natl Engn Res Ctr Vegetables, Beijing 100097, Peoples R China
3.Beijing Key Lab Vegetable Germplasm Improvement, Beijing 100097, Peoples R China
关键词： bacterial pathogens; bioinformatics; microbe-genome sequencing
期刊名称：PHYTOPATHOLOGY （ 影响因子：3.2； 五年影响因子：3.9 ）
ISSN： 0031-949X
年卷期： 2022 年 112 卷 3 期
页码：
收录情况： SCI
摘要： Bacterial wilt, caused by Ralstonia solanacearum, is a serious disease in pepper. However, the interaction between the pathogen and pepper remains largely unknown. This study aimed to gain insights into determinants of pepper susceptibility and R. solanacearum pathogenesis. We assembled the complete genome of R. solanacearum strain Rs-SY1 and identified 5,106 predicted genes, including 84 type III effectors (T3E). RNA-seq was used to identify differentially expressed genes (DEGs) in susceptible pepper CM334 at 1 and 5 days postinoculation (dpi) with R. solanacearum. Dual RNA-seq was used to simultaneously capture transcriptome changes in the host and pathogen at 3 and 7 dpi. A total of 1,400, 3,335, 2,878, and 4,484 DEGs of pepper (PDEGs) were identified in the CM334 hypocotyls at 1, 3, 5, and 7 dpi, respectively. Functional enrichment of the PDEGs suggests that inducing ethylene production, suppression of photosynthesis, downregulation of polysaccharide metabolism, and weakening of cell wall defenses may contribute to successful infection by R. solanacearum. When comparing in planta and nutrient agar growth of the R. solanacearum, 218 and 1,042 DEGs of R. solanacearum (RDEGs) were detected at 3 and 7 dpi, respectively. Additional analysis of the RDEGs suggested that enhanced starch and sucrose metabolism, and upregulation of virulence factors may promote R. solanacearum colonization. Strikingly, 26 R. solanacearum genes were found to have similar DEG patterns during a variety of host-R. solanacearum interactions. This study provides a foundation for a better understanding of the transcriptional changes during pepper-R. solanacearum interactions and will aid in the discovery of potential susceptibility and virulence factors.
- 相关文献
作者其他论文 更多>>
-
Comparative Transcriptomics Analysis Reveals the Differences in Transcription between Resistant and Susceptible Pepper (Capsicum annuum L.) Varieties in Response to Anthracnose
作者：Wang, Yixin;Chen, Bin;Cheng, Chunyuan;Du, Heshan;Geng, Sansheng;Zhang, Xiaofen;Fu, Bingkun;Qi, Meixia
关键词：pepper; anthracnose; GO enrichment; Colletotrichum capsici; transcription
-
First Report of Broad Bean Wilt Virus 2 Infecting Balsam (Impatiens balsamina) in China
作者：Qiu, Yanhong;Zhang, Haijun;Wang, Kejun;Wang, Hongyan;Wang, Dexin;Xia, Yang;Wen, Changlong;Xu, Xiulan;Qiu, Yanhong;Zhang, Haijun;Wang, Hongyan;Wang, Dexin;Xia, Yang;Wen, Changlong;Xu, Xiulan;Wang, Kejun
关键词：balsam; broad bean wilt virus 2; Impatiens balsamina; small RNA sequencing
-
Transcriptomic and metabolomic analysis of the delay in chilling injury in green bell peppers (Capsicum annuum L.) by sodium hexametaphosphate
作者：Wang, Xuanqi;Lv, Jieyu;Xu, Xiangbin;Wang, Xuanqi;Yuan, Shuzhi;Shi, Junyan;Lv, Jieyu;Zhao, Yaqi;Lu, Hongshan;Zuo, Jinhua;Chen, Bin;Wang, Qing;Lu, Hongshan
关键词：Green bell pepper fruit; Chilling injury; Sodium hexametaphosphate; Transcriptomic profiling; Metabolite
-
Optimized Pepper Target SNP-Seq Applied in Population Structure and Genetic Diversity Analysis of 496 Pepper (Capsicum spp.) Lines
作者：Wang, Yihao;Chen, Bin;Geng, Sansheng;Zhang, Xiaofen;Yang, Jingjing;Zhang, Jian;Du, Heshan;Chen, Bin;Zhang, Jian;Li, Wenyue
关键词：pepper; target SNP-seq; fruit shape; genetic diversity; population structure
-
Tuta absoluta management in China: progress and prospects
作者：Wang, Ming-hui;Ismoilov, Khasan;Zhang, Li-yun;Han, Peng;Wang, Ming-hui;Ismoilov, Khasan;Han, Peng;Liu, Wan-xue;Zhang, Yibo;Bai, Ming;Bai, Ming;Bai, Ming;Bai, Xiao-shuan;Chen, Bin;Gui, Fu-rong;Zhang, Xiao-ming;Chen, Haoliang;Chen, Hong-song;Dong, Yong-cheng;Fang, Kui;Huang, Guo-Hua;Jiang, Chun-mei;Jiang, Hong-bo;Jiang, Hong-bo;Li, Xiao-wei;Lu, Yao-bin;Luo, Chen;Luo, Chen;Lu, Zhao-zhi;Lu, Yao-bin;Ma, De-ying;Pu, De-qiang;Qu, Yanyan;Sang, Wen;Song, Li-mei;Sun, Xiao;Sun, Yuan-xing;Wan, Bin;Wang, Xin-pu;Yang, Wen-jia;Yang, Xue-qing;Yang, Xue-qing;Yao, Feng-Luan;Ye, Zheng-pei;Zhang, Ye;Zhu, Wen-ya;Zhao, Chen-chen;Zhou, Qiong;Zhou, Wen-wu;Rodriguez-Saona, Cesar;Biondi, Antonio;Jaworski, Coline C.;Desneux, Nicolas
关键词：biological invasion; insect pests; IPM; multi-pest approach; international projects; South American tomato pinworm
-
A comprehensive physiological and -Omic analysis of trypsin-mediated protection of green pepper fruits from chilling injury
作者：Zhao, Keyan;Wang, Xuanqi;Yue, Xiaozhen;Lv, Jieyu;Xu, Xiaodi;Lu, Hongshan;Zuo, Jinhua;Chen, Bin;Yuan, Shuzhi;Wang, Qing;Wang, Xuanqi;Lv, Jieyu;Xu, Xiangbin
关键词：Carotenoid biosynthesis; Capsicum annuum; Malondialdehyde; MAPK; Metabolome; Sugar metabolism; Transcriptome
-
A long noncoding RNA functions in pumpkin fruit development through S-adenosyl-
l -methionine synthetase作者：Tian, Jiaxing;Zhang, Fan;Zhang, Guoyu;Li, Xiaojie;Wen, Changlong;Li, Haizhen;Tian, Jiaxing;Zhang, Fan;Zhang, Guoyu;Li, Xiaojie;Wen, Changlong;Li, Haizhen
关键词：
