文献类型： 外文期刊

作者： Ye, Deyou ¹ ; Qi, Yonghong ² ; Cao, Sufang ³ ; Duan, Yanqiao ¹ ; Huynh, Bao-Lam ⁴ ;

作者机构： 1.Gansu Acad Agr Sci, Inst Vegetables, Lanzhou 730070, Gansu, Peoples R China

2.Gansu Acad Agr Sci, Inst Plant Protect, Lanzhou 730070, Gansu, Peoples R China

3.Gansu Acad Agr Sci, Inst Fruit & Floriculture, Lanzhou 730070, Gansu, Peoples R China

4.Univ Calif Riverside, Dept Nematol, Riverside, CA 92521 USA

关键词： Identification; Characterization; microRNA396; Targets; Cucumis metuliferus; Meloidogyne incognita

期刊名称：SOUTH AFRICAN JOURNAL OF BOTANY （ 影响因子：3.1； 五年影响因子：3.1 ）

ISSN： 0254-6299

年卷期： 2024 年 165 卷

页码：

收录情况： SCI

摘要： MicroRNAs (miRNAs), a class of non -coding small RNAs, play an important role in biotic and abiotic stresses. Previously, great progress has been made in miRNA-guided gene regulation in plant -parasitic cyst nematodes interactions. However, little is known about miRNAs regulation of nematode stress response in Cucumis metuliferus, which is a relative of cultivated cucumber or melon and highly resistant to the root -knot nematode Meloidogyne incognita. On the basis of our investigation recently, five cmu-miR396 precursors were firstly identified and characterized from C. metuliferus in this study. Subquently, a total of 12 genes were predicted to be as the potential targets of cmu-miR396 and their function involved in transcription factor, metabolism, signal transduction and stress response. Moreover, three cmu-GRFs genes with significant differential expression in roots of C. metuliferus were successfully amplified and confirmed by RT-PCR experimently. Additionally, the analysis was performed for the phylogeny and subcellular localization of cmu-GRFs protein, and the three transcription factors cmu-GRFs were confirmed as the candidate targets of cmumiR396 through 50RLM-RACE assays. These data laid a foundation for further functional analyses to reveal the roles played by miR396 during the interaction between C. metuliferus and M. incognita, leading to a better understanding of the mechanisms and underlying regulatory networks of miR396-mediated resistance to M. incognita in C. metuliferus. (c) 2024 SAAB. Published by Elsevier B.V. All rights reserved.