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The identification and expression of an interleukin-21 receptor in large yellow croaker (Larimichthys crocea)

文献类型： 外文期刊

作者： Wu, Hanyu 1 ; Fu, Qiuling 3 ; Teng, Yan 1 ; Mu, Pengfei 1 ; Chen, Jingjie 1 ; Chen, Xinhua 1 ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Marine Sci, State Key Lab Mariculture Breeding, Key Lab Marine Biotechnol Fujian Prov, 15 Shangxiadian Rd, Fuzhou 350002, Peoples R China

2.Southern Marine Sci & Engn Guangdong Lab Zhuhai, Zhuhai 519000, Peoples R China

3.Fujian Acad Agr Sci, Inst Anim Husb & Vet Med, Fuzhou 350013, Peoples R China

关键词： Larimichthys crocea; IL-21R; IL-21; Immune response

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.8； 五年影响因子：2.7 ）

ISSN： 0301-4851

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Background: We identified a homologue of IL-21R (LcIL-21R) in large yellow croaker (Larimichthys crocea, Lc). Our investigation focused on understanding the molecular structural features and immune function of LcIL-21R.Methods: We cloned the LcIL-21R gene from the genome of Larimichthys crocea by RT-PCR, and the molecular and structural characteristics of LcIL-21R were analyzed by a series of protein analysis tools. We used real-time PCR to investigate the tissue distribution of LcIL-21R, and LcIL-21R gene expression regulation was also measured in head kidney leukocytes under trivalent bacterial vaccine or poly (I:C) stimulation.Results: The open reading frame (ORF) of the LcIL-21R gene is 1629 bp long and encodes a precursor protein of 542 amino acids (aa), with a 23-aa signal peptide and a 519-aa mature peptide containing four putative N-glycosylation sites. LcIL-21R has two fibronectin type III (FNIII)-like domains (D1 and D2), a transmembrane domain, and a cytoplasmic region. A conserved WSXWS motif was also found in the D2 domain. The predicted structure of the extracellular region of LcIL-21R (LcIL-21R-Ex) is highly similar to that of human IL-21R. LcIL-21R was constitutively expressed in all tissues examined, and LcIL-21R mRNA levels were increased in the head kidney and spleen upon inactivated trivalent bacterial vaccine or poly(I:C) stimulation.Conclusions: Our results suggest that LcIL-21R shares structural and functional properties with IL-21Rs found in other vertebrates, indicating its potential involvement in the IL-21-mediated immune response to pathogenic infections. These findings contribute to our understanding of the evolutionary conservation of IL-21 signaling and its role in the immune system.

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