文献类型： 外文期刊

作者： Ye, Xiaoyun ¹ ; Wang, Mina ¹ ; Zhao, Liqun ⁴ ; Wang, Zhiqiang ¹ ; Yu, Lingda ¹ ; Yuan, Shuzhi ¹ ; Xu, Xiaodi ¹ ; Xu, Xiangbin ² ; Zuo, Jinhua ¹ ; Chen, Qing ⁷ ; Feng, Bihong ³ ; Yue, Xiaozhen ¹ ; Wang, Qing ¹ ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Key Lab Biol & Genet Improvement Hort Crops North, Key Lab Urban Agr North Minist Agr, Inst Agrifood Proc & Nutr,Key Lab Vegetable Postha, Beijing 100097, Peoples R China

2.Hainan Univ, Sch Food Sci & Engn, Key Lab Food Nutr & Funct Food Hainan Prov, Haikou 570228, Peoples R China

3.Guangxi Univ, Coll Agr, Nanning 530004, Peoples R China

4.Beijing Agr Technol Extens Stn, Beijing 100029, Peoples R China

5.Anhui Agr Univ, Sch Life Sci, Hefei 230036, Peoples R China

6.Chongqing Univ Arts & Sci, Chongqing 402160, Peoples R China

7.China Agr Univ, Coll Resources & Environm Sci, Beijing Key Lab Farmyard Soil Pollut Prevent Contr, Beijing 100193, Peoples R China

关键词： Chlorophyll; Transcriptional metabolic analysis; Senescence; Postharvest; Chlorophyll; Transcriptional metabolic analysis; Senescence; Postharvest

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）

ISSN： 0141-8130

年卷期： 2025 年 311 卷

页码：

收录情况： SCI

摘要： Lactase peptide maintains food quality and ectoine stabilizes cell structure and function. The present study investigated the synergistic effect of 0.5 % lactopeptide and 0.1 % ectoine on preserving the quality of pak choi stored at 20 degrees C and 90 % relative humidity (RH) for 6 d. Results indicated that the combined treatment significantly reduced weight loss, electrolyte leakage, respiration rate, and ethylene generation compared to the untreated control or pak choi treated with ectoine. Furthermore, the combined treatment enhanced the level of ascorbic acid and flavonoids, and increased superoxide dismutase (SOD) activity. A combined transcriptomic and metabolomic analysis revealed the upregulation of key genes encoding proteins involved in the chlorophyll biosynthesis pathway, including protochlorophyllide reductase (POR), divinyl chlorophyllide a 8-vinyl-reductase (DVR), and chlorophyll/bacteriochlorophyll a synthase (CHLG), while the expression of the gene encoding a chlorophyll-degrading enzyme, chlorophyll (ide) b reductase (NOL), was suppressed. The combined treatment increased the abundance of metabolites associated with the citrate cycle pathway, including L-glutamate, Lglutamine, and L-asparagine. Additionally, the expression of chalcone isomerase (4CL), naringenin 3-dioxygenase (F3H) and chalcone synthase (CHS) in the phenylalanine, tyrosine, and tryptophan biosynthesis pathways, was elevated compared to the untreated control, resulting in increased levels of luteolin and naringenin chalcone. Collectively, our results indicate that the combined use of ectoine and lactopeptide effectively delayed the yellowing and postharvest senescence of pak choi during storage. The present study provides a foundation for further investigations of the postharvest metabolism of pak choi.