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Exogenous indole modulates several CpxRA-mediated virulence-related parameters of Edwardsiella piscicida in vitro

文献类型： 外文期刊

作者： Gou, Yin 1 ; Fang, Qingjian 1 ; Gu, Hanjie 2 ; Tian, Aijun 2 ; Li, Jun 5 ; Wang, Yan 1 ; Hu, Yonghua 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Natl Key Lab Trop Crop Breeding, Zhanjiang Expt Stn, Sanya 572024, Peoples R China

2.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Haikou 571101, Peoples R China

3.Qingdao Marine Sci & Technol Ctr, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China

4.Chinese Acad Trop Agr Sci, Sanya Res Inst, Sanya 572025, Peoples R China

5.Lake Super State Univ, Sch Sci & Med, Sault Ste Marie, MI 49783 USA

6.Hainan Prov Key Lab Funct Components Res & Utiliza, Haikou 571101, Peoples R China

7.Huazhong Agr Univ, Coll Fisheries, Dept Aquat Anim Med, Wuhan 430070, Peoples R China

8.Ningbo Univ, Sch Marine Sci, Ningbo 315211, Peoples R China

关键词： Edwardsiella piscicida; Indole; CpxRA; Stress resistance; Virulence

期刊名称：MARINE LIFE SCIENCE & TECHNOLOGY （ 影响因子：5.8； 五年影响因子：5.7 ）

ISSN： 2096-6490

年卷期： 2024 年

页码：

收录情况： SCI

摘要： Indole signaling has been regarded as a promising target to control aquatic diseases. However, the relationship between exogenous indole and the virulence of Edwardsiella piscicida is obscure. E. piscicida is a facultative intracellular pathogen, and has been a model strain in aquaculture. In this study, we investigated the effect of exogenous indole on stress resistance and virulence of E. piscicida in the presence of and absence of endogenous indole (WT and Delta tnaA, respectively). Our results showed that exogenous indole reduced the resistance of WT against strong acidic stress, but enhanced that of Delta tnaA. Also, we found that exogenous indole abated viability of E. piscicida at high temperature, repressed bacterial biofilm formation, impaired bacterial envelope integrity, and weakened bacterial proliferation in macrophages, irrespective of the presence or absence of endogenous indole. These virulence-related phenotypes caused by exogenous indole are reasonably explained by the observation that exogenous indole downregulated the expressions of CpxRA and its target YccA via being responded by CpxA. The effects of exogenous indole on strong acid resistance are partially achieved by changing the expression of GadD, the key functional enzyme of acid resistance system (AR2). We believe that this is the first report about the impact of exogenous indole on strong acid stress and membrane integrity of pathogenic bacteria. Also, we reveal the likely mechanism by which exogenous indole regulates the expressions of virulence-related genes. These findings provide a new understanding on pathogenesis of E. piscicida and contribute to the prevention and control strategies of edwardsiellosis.

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