Molecular basis of cross-resistance in Sagittaria trifolia L. against acetolactate-synthase inhibitors
文献类型： 外文期刊
作者： Fu, Danni 1 ; Zhao, Bochui 2 ; Li, Xiuwei 3 ; Wei, Songhong 3 ; Ji, Mingshan 3 ;
作者机构： 1.Inner Mongolia Univ Nationalities, Tongliao 028000, Peoples R China
2.Hebei Acad Agr & Forestry Sci, Inst Cereal & Oil Crops, Shijiazhuang 050035, Hebei, Peoples R China
3.Shenyang Agr Univ, Coll Plant Protect, Shenyang 110866, Peoples R China
关键词： target-site mutation; genetic analysis; sulfonylurea; triazolopyrimidine; pyrimidinyl-thiobenozoate
期刊名称：SCIENCEASIA （ 影响因子：0.615； 五年影响因子：0.708 ）
ISSN： 1513-1874
年卷期： 2020 年 46 卷 5 期
页码：
收录情况： SCI
摘要： Herbicide resistance to sulfonylureas in Sagittaria trifolia L. is a common problem in northern China. In our study, we used whole-plant dose-response, acetolactate synthase (ALS) sequencing, and ALS enzyme activity methods to assess 3 putative resistant (LN-1, LN-2, and LN-3) and one susceptible (S) S. trifolia populations for crossresistance to ALS inhibitors (bensulfuron-methyl, ethoxysulfuron, pyrazosulfuron-ethyl, penoxsulam, pyribenzoxim, and bispyribac-sodium). Regarding the whole-plant dose-response and the in vitro ALS assays, the results showed that the LN-2 population evolved cross-resistance to all herbicides tested whereas the other two populations showed crossresistance only to bensulfuron-methyl, ethoxysulfuron, pyrazosulfuron-ethyl, and penoxsulam. Results of the in vitro ALS assays were consistent with whole-plant dose-response data. The DNA sequencing of the ALS gene showed that LN-1 and LN-3 populations had a single nucleotide polymorphism in the Pro 197 codon, resulting in the substitution of proline (Pro) by serine (Ser) and leucine (Leu), respectively. However, the LN-2 population showed single nucleotide polymorphism in the second position of the Asp 376 codon, causing substitution of aspartic acid (Asp) by glutamic acid (Glu). The cross-resistance of the S. trifolia population to all ALS inhibitors due to Asp 376 mutation in the ALS gene of this species is reported for the first time.
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