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An easy and robust method for the isolation of high quality RNA from coconut tissues

文献类型： 外文期刊

作者： Iqbal, Amjad 1 ; Yang, Yaodong 1 ; Wu, Yi 1 ; Li, Jing 1 ; Hamayun, Muhammad 3 ; Hussain, Anwar 3 ; Shah, Farooq 4 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Hainan Key Lab Trop Oil Crops Biol, Coconut Res Inst, Wenchang 571339, Hainan, Peoples R China

2.Abdul Wali Khan Univ Mardan, Dept Food Sci & Technol, Mardan 23200, Pakistan

3.Abdul Wali Khan Univ Mardan, Dept Bot, Mardan 23200, Pakistan

4.Abdul Wali Khan Univ Mardan, Dept Agron, Mardan 23200, Pakistan

关键词： cDNA library; Coconut tissues; Complex matrices; High quality RNA; RNA extraction; RT-PCR

期刊名称：ELECTRONIC JOURNAL OF BIOTECHNOLOGY （ 影响因子：2.8； 五年影响因子：3.379 ）

ISSN： 0717-3458

年卷期： 2020 年 48 卷

页码：

收录情况： SCI

摘要： Background: Coconut tissues consist of a complex network of polysaccharides, proteins, polyphenols, and lipids that can bind to nucleic adds and pose difficulty in isolation. Certainly, a vigorous method is required to isolate high quality and quantity of RNA from such tissues for the purpose of downstream experiments. In this paper, we discuss a newly developed method for the Isolation of RNA from Complex Matrices (IRCM) method from coconut tissues. Results: The method is robust, cheap, and efficient for the extraction of quality RNA in high quantities from the solid endosperm of stored and fresh coconut (150 mu g/g FW with A260/280 = 1.89 and 247.5 mu g/g FW with A260/280 = 1.91). coconut apple (263.8 mu g/g FW with A260/280 = 1.97). and coconut bud (1052.5 mu g/g FW with A260/280 = 2.00). The other well established methods, such as Method of RNA Isolation from Palm (MRIP), Cetyl Trimethyl Ammonium Bromide (CTAB), TRIZOL, and RNA plant kit failed to isolate quality RNA in appreciable quantities from the coconut tissues. Furthermore, the resultant RNA performed well in the downstream experiment, that is, RT-PCR for the production and amplification of cDNA. Conclusions: From the study, we conduded that the present method will play a vital role in the extraction of high quality RNA from complex matrices in a short time. (C) 2020 Pontificia Universidad Catolica de Valparaiso. Production and hosting by Elsevier B.V. All rights reserved.

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