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Identification and Functional Characterization of a Sigma Glutathione S-Transferase CpGSTs2 Involved in lambda-Cyhalothrin Resistance in the Codling Moth Cydia pomonella

文献类型： 外文期刊

作者： Hu, Chao 1 ; Wei, Zi-Han 1 ; Li, Pei-Rong 1 ; Harwood, James D. 3 ; Li, Xiang-Yang 4 ; Yang, Xue-Qing 1 ;

作者机构： 1.Shenyang Agr Univ, Coll Plant Protect, Shenyang 110866, Liaoning, Peoples R China

2.Key Lab Econ & Appl Entomol Liaoning Prov, Shenyang 110866, Liaoning, Peoples R China

3.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing 100097, Peoples R China

4.Guizhou Univ, State Key Lab Breeding Base Green Pesticide & Agr, Minist Educ, Key Lab Green Pesticide & Agr Bioengn, Guiyang, Peoples R China

关键词： Cydia pomonella; glutathione S-transferases; sigma-class; insecticide resistance; metabolism

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2020 年 68 卷 45 期

页码：

收录情况： SCI

摘要： The codling moth, Cydia pomonella (L.), is a quarantine pest of global significance impacting pome fruits and walnuts. It has evolved resistance to many commonly used insecticides including lambda-cyhalothrin. Glutathione S-transferases (GSTs) are multifunctional enzymes playing a crucial role in the detoxification of insecticides in insects. However, the role of specific GST gene in lambda-cyhalothrin resistance in C. pomonella is unclear. In this study, we identified three sigma-class genes (CpGSTs1, CpGSTs2, and CpGSTs3). These genes were ubiquitously expressed at all developmental stages, and of these, the expression level of CpGSTs2 in the larval stage was significantly higher than in the egg, pupal, and adult stages. Moreover, CpGSTs2 was predominantly expressed in the fat body while lower levels in the cuticle. In addition to exposure of larvae to LD10 of lambda-cyhalothrin elevating the expression level of CpGSTs2, mRNA levels of CpGSTs2 in a field population (ZW_R) from northeast China, which has developed moderate level resistance to lambda-cyhalothrin, was significantly higher than that of susceptible strains. In vitro inhibition assays demonstrated that lambda-cyhalothrin inhibited the conjugating activities of recombinant CpGSTs2, and metabolic assays indicated that lambda-cyhalothrin could be depleted by recombinant CpGSTs2. These results bring evidence for the involvement of CpGSTs2 in C. pomonella in resistance to lambda-cyhalothrin.

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