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Characterization and complete genome sequences of two novel variants of the family Closteroviridae from Chinese kiwifruit

文献类型： 外文期刊

作者： Feng, Xin 1 ; Lai, Rui-lian 1 ; Gao, Min-xia 1 ; Chen, Wen-guang 1 ; Wu, Ru-jian 1 ; Cheng, Chun-zhen 3 ; Chen, Yi-tin 1 ;

作者机构： 1.Fujian Acad Agr Sci, Fruit Res Inst, Fuzhou, Fujian, Peoples R China

2.Res Ctr Engn Technol Fujian Deciduous Fruits, Fuzhou, Fujian, Peoples R China

3.Fujian Agr & Forestry Univ, Fuzhou, Fujian, Peoples R China

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2020 年 15 卷 11 期

页码：

收录情况： SCI

摘要： Two distinct closterovirus-like genome sequences (termed AdV-1 v1 and v2) were identified in Actinidia chinensis var. deliciosa 'Miliang-1' that had no disease symptoms using high-throughput sequencing. Using overlapping reverse transcription-polymerase chain reaction and rapid amplification of cDNA ends, the genomic sequences of AdV-1 v1 and v2 were confirmed as 17,646 and 18,578 nucleotides in length, respectively. The two complete genomes contained 9 and 15 open reading frames, respectively, coding for proteins having domains typical of Closteroviridae, such as RNA-dependent RNA polymerase (RdRp), heat shock protein 70 homolog (HSP70h) and coat protein (CP). Sequence analysis showed that the amino acid sequences of RdRp, HSP70h, and CP of the two variants exhibited high similarity (> 80%), while their genomic organization was somewhat different. This suggested that the two viral genomes identified here are variants of the family Closteroviridae in a single kiwifruit host. Furthermore, phylogenetic relationship analysis revealed that the two variants had a closer relationship with the unclassified virus Persimmon virus B (PeVB) and Actinidia virus 1 (AcV-1) than with other members of the family Closteroviridae, as did their genomic organization. It is speculated that the two variants, together with PeVB and AcV-1 belong to a new subfamily of Closteroviridae.

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