文献类型： 外文期刊
作者： Na Zhao 1 ; Bo Jiang 2 ; Yifei Yang 3 ; Fuyuan Cao 1 ; Bin He 1 ; Yanshu Zhang 1 ; Huan Jin 2 ; Jingliang Su 4 ; Shuang Li 1 ;
作者机构： 1.North China Univ Sci & Technol, Expt Anim Ctr, Tangshan, Peoples R China
2.Beijing Acad Agr & Forestry Sci, Inst Anim Husb & Vet Med, Beijing, Peoples R China
3.China Acad Chinese Med Sci, Inst Chinese Mat Med, Beijing, Peoples R China
4.China Agr Univ, Coll Vet Med, Key Lab Anim Epidemiol & Zoonosis, Minist Agr, Beijing, Peoples R China
关键词： murine norovirus; virus isolate; persistent infection; laboratory animals; C57 (C57BL; 6J)
期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：3.412； 五年影响因子：3.588 ）
ISSN：
年卷期： 2020 年 7 卷
页码：
收录情况： SCI
摘要： Murine Norovirus (MNV) is one of the most known viruses among viruses in mice. Because of the high prevalence of MNV in frequently used laboratory animals in biomedical researches, there is a significant impact of MNV. There may be different prevalence degrees and molecular characteristics of MNV in different regions around the world. Here, we reported an MNV strain "designated HBTS-1806" isolation from commercial mice's feces that caused a detectable cytopathic effect (CPE) in RAW264.7 cells. According to electron microscopy, the virus was 50-70 nm in diameter. The complete genome of HBTS-1806 is 7383 nucleotides with a structure similar to that of MNV reference strains. According to phylogenetic analysis on the basis of the whole genome, HBTS-1806 shared nucleotide sequence identities of 90.2-95.4% with other Chinese isolates reported. Analysis of amino acid sequence on the basis of ORF1 and ORF2 suggested that the isolated strain may be derived from recombination. Although no gross lesions or histopathological changes were found from mice infected with 5 x 10(5) TCLD50 of MNV by oral gavage inoculation, the intestinal virus loads lasted 12 weeks, suggesting a persistent infection strain of MNV isolate in China.
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