您好，欢迎访问北京市农林科学院 机构知识库！
北京市农林科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Chromosome-level genome assembly of the aphid parasitoid Aphidius gifuensis using Oxford Nanopore sequencing and Hi-C technology

文献类型： 外文期刊

作者： Li, Bingyan 1 ; Du, Zhenyong 1 ; Tian, Li 1 ; Zhang, Limeng 3 ; Huang, Zhihua 3 ; Wei, Shujun 4 ; Song, Fan 1 ; Cai, Wanz 1 ;

作者机构： 1.China Agr Univ, Coll Plant Protect, Dept Entomol, Beijing 100193, Peoples R China

2.China Agr Univ, Coll Plant Protect, MOA Key Lab Pest Monitoring & Green Management, Beijing 100193, Peoples R China

3.Tobacco Co, Yuxi 653100, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing, Peoples R China

5.China Natl Tobacco Corp, Yunnan Tobacco Co, Kunming 650011, Yunnan, Peoples R China

关键词： Aphidius gifuensis; chromosome-level genome; Hi-C; nanopore; parasitoid wasp

期刊名称：MOLECULAR ECOLOGY RESOURCES （ 影响因子：7.09； 五年影响因子：8.745 ）

ISSN： 1755-098X

年卷期： 2021 年 21 卷 3 期

页码：

收录情况： SCI

摘要： Aphidius gifuensis is a parasitoid wasp that has been commercially bred and released in large scale as a biocontrol agent for the management of aphid pests. As a highly efficient endoparasitoid, it is also an important model for exploring mechanisms of parasitism. Currently, artificially bred populations of this wasp are facing rapid decline with undetermined cause, and mechanisms underlying its parasitoid strategy remain poorly understood. Exploring the mechanism behind its population decline and the host-parasitoid relationship is impeded partly due to the lack of a comprehensive genome data for this species. In this study, we constructed a high-quality reference genome of A. gifuensis using Oxford Nanopore sequencing and Hi-C (proximity ligation chromatin conformation capture) technology. The final genomic assembly was 156.9 Mb, with a contig N50 length of 3.93 Mb, the longest contig length of 10.4 Mb and 28.89% repetitive sequences. 99.8% of genome sequences were anchored onto six linkage groups. A total of 11,535 genes were predicted, of which 90.53% were functionally annotated. Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis showed the completeness of assembled genome is 98.3%. We found significantly expanded gene families involved in metabolic processes, transmembrane transport, cell signal communication and oxidoreductase activity, in particular ATP-binding cassette (ABC) transporter, Cytochrome P450 and venom proteins. The olfactory receptors (ORs) showed significant contraction, which may be associated with the decrease in host recognition. Our study provides a solid foundation for future studies on the molecular mechanisms of population decline as well as host-parasitoid relationship for parasitoid wasps.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：北京市农林科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台