PEGylated nano-Rehmannia glutinosa polysaccharide induces potent adaptive immunity against Bordetella bronchiseptica
文献类型： 外文期刊
作者： Huang, Yee 1 ; Nan, Li 2 ; Xiao, Chenwen 1 ; Su, Fei 1 ; Li, Ke 1 ; Ji, Quan-An 1 ; Wei, Qiang 1 ; Liu, Yan 1 ; Bao, Guolian 1 ;
作者机构： 1.Zhejiang Acad Agr Sci, Inst Anim Husb & Vet Sci, Hangzhou 310021, Peoples R China
2.Zhejiang Normal Univ, Jinhua 321000, Zhejiang, Peoples R China
关键词： Rehmannia glutinosa polysaccharide; PEGylation; Bordetella bronchiseptica; Dendritic cells; Adaptive immunity
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2021 年 168 卷
页码：
收录情况： SCI
摘要： Vaccines, in many cases, stimulate only too weak immunogenicity to prevent infection. Therefore, adjuvants are required during their preparation to boost the immune response. We herein developed a PEGylated nanoadjuvant based on Rehmannia glutinosa polysaccharide (RGP). The addition of PEG layer exhibits enhanced immune performance of the nano-RGP. Stimulation of dendritic cells (DCs) with PEGylated nano-RGP (pRL) led to increased proliferation and cytokine production (IL-6, IL-12, IL-1 beta and TNF-alpha). The pRL was internalized into DCs via a rapid and efficient method. The mice immunized with pRL exhibited enhanced antigen-specific serum IgG and Th1-(IFN-gamma), Th2-(IL-4), and Th17-( IL-17, IL-6) cytokine production, contributing to a good anti-infection performance. Furthermore, the pRL could effectively deliver the antigen to the lymph nodes (LNs), activate DC in the LN and produce enhanced CD4(+) and CD8(+) T-cells-derived memory (CD44(high) CD62L(high)), and effector (CD44(high) CD62L(low)) as well as functional phenotypes. Our results revealed that pRL can act as a promising adjuvant with targeted delivery of antigen due to its effective activation and robust adaptive immunity induction of DCs. (c) 2020 Elsevier B.V. All rights reserved.
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