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Heterologous expression of Arabidopsis thaliana rty gene in strawberry (Fragaria x ananassa Duch.) improves drought tolerance

文献类型： 外文期刊

作者： Li, Maofu 1 ; Yang, Yuan 1 ; Raza, Ali 4 ; Yin, Shanshan 1 ; Wang, Hua 1 ; Zhang, Yuntao 1 ; Dong, Jing 1 ; Wang, Guixia; 1 ;

作者机构： 1.Beijing Acad Agr & Forestry Sci, Beijing Acad Forestry & Pomol Sci, Beijing 100093, Peoples R China

2.Minist Agr, Key Lab Biol & Genet Improvement Hort Crops North, Beijing 100093, Peoples R China

3.Beijing Engn Res Ctr Deciduous Fruit Trees, Beijing 100093, Peoples R China

4.Chinese Acad Agr Sci CAAS, Key Lab Biol & Genet Improvement Oil Crops, Oil Crops Res Inst, Wuhan 430062, Peoples R China

关键词： ABA; Arabidopsis thaliana; Drought stress; Heterologous expression; Stomatal closure; Strawberry

期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.215； 五年影响因子：4.96 ）

ISSN： 1471-2229

年卷期： 2021 年 21 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundStrawberry (Fragaria x ananassa Duch.) is an important fruit crop worldwide. It was particularly sensitive to drought stress because of their fibrous and shallow root systems. Mutant rty of Arabidopsis thaliana ROOTY (RTY) results in increased endogenous auxin levels, more roots, and shoot growth. It is still unclear whether the rty gene improves stress tolerance in strawberry.Resultsrty gene was isolated from Arabidopsis thaliana and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the pBI121-rty binary vector carrying the selectable marker of neomycin phosphotransferase II (NPT II). Seven transgenic lines were confirmed by PCR and western blot analysis. Accumulations of IAA and ABA were significantly increased in the transgenic plants. The endogenous IAA contents were 46.5ngg(-1) and 66.0ngg(-1)in control and transgenic plants respectively. The endogenous ABA contents in the control plant were 236.3ngg(-1) and in transgenic plants were 543.8ngg(-1). The production of adventitious roots and trichomes were enhanced in the transgenic plants. Furthermore, transcript levels of the genes including IAA and ABA biosynthetic, and stress-responsive genes, were higher in the transgenic plants than in the control plants under drought conditions. Water use efficiency and a reduced water loss rate were enhanced in the transgenic strawberry plants. Additionally, peroxidase and catalase activities were significantly higher in the transgenic plants than in the control plants. The experiment results revealed a novel function for rty related to ABA and drought responses.ConclusionsThe rty gene improved hormone-mediated drought tolerance in transgenic strawberry. The heterologous expression of rty in strawberry improved drought tolerance by promoting auxin and ABA accumulation. These phytohormones together brought about various physiological changes that improved drought tolerance via increased root production, trichome density, and stomatal closure. Our results suggested that a transgenic approach can be used to overcome the inherent trade-off between plant growth and drought tolerance by enhancing water use efficiency and reducing water loss rate under water shortage conditions.

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