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Quantification of Pseudomonas protegens FD6 and Bacillus subtilis NCD-2 in soil and the wheat rhizosphere and suppression of root pathogenic Rhizoctonia solani AG-8

文献类型： 外文期刊

作者： Zhang, Qingxia 1 ; Stummer, Belinda E. 2 ; Guo, Qinggang 3 ; Zhang, Wen 4 ; Zhang, Xinjian 4 ; Zhang, Liqun 5 ; Harvey 1 ;

作者机构： 1.Yangzhou Univ, Coll Hort & Plant Protect, Yangzhou 225009, Jiangsu, Peoples R China

2.CSIRO Agr & Food, PMB 2, Glen Osmond, SA 5064, Australia

3.Hebei Acad Agr & Forestry Sci, Inst Plant Protect, Baoding 071000, Hebei, Peoples R China

4.Qilu Univ Technol, Shandong Acad Sci, Ecol Res Inst, Jinan 250014, Shandong, Peoples R China

5.China Agr Univ, Dept Plant Pathol, Beijing 100094, Peoples R China

关键词： Pseudomonas; Bacillus; Wheat rhizosphere; Rhizoctonia solani; Biocontrol; qPCR

期刊名称：BIOLOGICAL CONTROL （ 影响因子：3.687； 五年影响因子：3.962 ）

ISSN： 1049-9644

年卷期： 2021 年 154 卷

页码：

收录情况： SCI

摘要： Bacillus subtilis strain NCD-2 and Pseudomonas protegens strain FD6 produce a range of antimicrobial metabolites known to be effective against soil-borne plant pathogens. Quantitative qPCR assays were designed from sequence characterized amplified fragment length polymorphisms (AFLP) diagnostic for each inoculant genotype. The qPCR minimum detection thresholds (MDT) for NCD-2 and FD6 ranged between 5.2 x 10(3) (log 3.71) and 1.5 x 10(6) (log 6.18) genome (cell) equivalents g(-1) of soil or wheat mot tissue and were independent of soil type. Wheat rhizosphere populations of strains FD6 and NCD-2 significantly declined during the first 14 days postinoculation and then remained stable for the next 14 days. Colonization of wheat mots, rhizosphere soil and bulk (nonrhizosphere) soil by FD6 was significantly greater than that of NCD-2. Strains FD6 and NCD-2 significantly decreased in vitro growth of wheat mot pathogenic Rhizoctonia solani AG-8, Pythium irregulare and Gaeumannomyces graminis var. tritici, fungal antibiosis by FD6 greater than NCD-2. Wheat rhizosphere colonization by NCD-2 and FD6 significantly decreased abundance of R. solani AG-8 in the rhizosphere by 3- and 13-fold respectively, pathogen suppression increasing (21%-29%) foliar biomass of wheat seedlings to levels not significantly different to the non-diseased control. The qPCR assays developed in this study were used to monitor soil and rhizosphere populations of P. protegens FD6 and B. subtilis NCD-2 and their suppressive efficacies against R. solani AG-8 in wheat. These assays can be applied to monitor the dynamics of these inoculants in suppressing Rhizoctonia root rot and other crop diseases.

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