Screening of sweet wampee [Clausena lansium (Lour.) Skeels] progenies in the early growth stage based on chloroplast genome analysis
文献类型： 外文期刊
作者： Zhao, Zhichang 1 ; Gao, Aiping 1 ; Huang, Jiangfeng 1 ; Luo, Ruixiong 1 ; Yu, Kaili 1 ;
作者机构： 1.Chinese Acad Trop Agr Sci, Trop Crops Genet Resources Inst, Natl Cultivar Improvement Ctr Trop Fruit Tree, Haikou 571101, Hainan, Peoples R China
2.Hainan Univ, Coll Hort, Haikou 570228, Hainan, Peoples R China
关键词： Molecular markers; PCR; Wampee genetic resources
期刊名称：GENETIC RESOURCES AND CROP EVOLUTION （ 影响因子：1.524； 五年影响因子：1.713 ）
ISSN： 0925-9864
年卷期： 2021 年 68 卷 5 期
页码：
收录情况： SCI
摘要： Wampee [Clausena lansium (Lour.) Skeels] was originally native to the southern part of China, and had a long history of cultivation. Wampee has been an important species in the development of the fruit breeding program in the genus Clausena, family Rutaceae. In addition to fruit characteristics and bark color, it is difficult to distinguish different varieties of wampee, more difficult of identification of seedling stage. In this study, we determined and analyzed the complete chloroplast genome of Jixin, seedless, and sweet wampee varieties. By comparison, it was found that the sweet wampee is 150 bp less than the other two varieties. We designed a pair of specific primers to distinguish between sweet wampee and other varieties, and it also has better discriminating ability through the analysis of seedlings. The complete chloroplast genome will provide valuable information for the development of highly variable DNA markers for future variety identification and population analysis studies of wampee.
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