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Conformational modifications of lysozyme caused by interaction with humic acid studied with spectroscopy

文献类型： 外文期刊

作者： Li, Yan 1 ; Koopal, Luuk K. 3 ; Chen, Yi 1 ; Shen, Alin 1 ; Tan, Wenfeng 4 ;

作者机构： 1.Zhejiang Acad Agr Sci, Inst Environm Resource & Soil Fertilizer, Hangzhou 310021, Peoples R China

2.Natl Agr Expt Stn Soil Qual, Jiaxing 314000, Peoples R China

3.Wageningen Univ & Res, Phys Chem & Soft Matter, Stippeneng 4, NL-6708 WE Wageningen, Netherlands

4.Huazhong Agr Univ, Coll Resources & Environm, Wuhan 430070, Peoples R China

关键词： Humic acid (HA); Lysozyme (LSZ); Mass ratio of HA/LSZ (f); pH; HA/ISZ-complexes/aggregates (HA/LSZ-c/a); Conformational modification

期刊名称：SCIENCE OF THE TOTAL ENVIRONMENT （ 影响因子：7.963； 五年影响因子：7.842 ）

ISSN： 0048-9697

年卷期： 2021 年 768 卷

页码：

收录情况： SCI

摘要： Modification of enzyme/protein conformation will affect the activities and functionality of enzymes. Previous studies have shown that the activity of lysozyme (LSZ) in the presence of humic acid (HA) is largely determined by the mass ratio of HA/LSZ (f = mHA/mLSZ), pH and ionic strength. Here the interaction and conformation of LSZ in HA/LSZ complex/aggregate (HA/LSZ c/a) were investigated by spectroscopic techniques at (initial) pH 5 and 8 and ionic strength 5 mmol/L. The results indicated a strong interaction between HA and LSZ Circular dichroism (CD), and attenuated total reflectance Fourier transform infrared (ATR-FTIR) spectroscopy showed that the helix content reached a minimum at the mass ratio of its iso electric point (LEP) at given initial pH, f(IEP,pHi). The changes in beta-sheet and random coil of HA/ISZ-c/a were opposite with increasing f. The minimum of helix content at f(IEP,pHi) corresponded with the minimum LSZ activity and maximum aggregate size of HA/LSZ c/a. UV vis spectra and fluorescence measurements indicated that the amino add residues (especially for tyrosine) in ISZ were in a more hydrophobic microenvironment before f(IEP,pHi) due to the formation of HA/LSZ-c/a, while were gradually exposed to a more polar microenvironment beyond f(IEP,pHi) with the disaggregation of HA/LSZ-c/a. HA and ISZ interaction caused a more hydrophobic microenvironment for the amino acid residues at initial pH 8. This study improves our understanding of enzyme/protein behavior in the natural environment (C) 2021 Elsevier B.V. All rights reserved.

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