Simultaneous determination of six glycosidic aroma precursors in pomelo by ultra-high performance liquid chromatography-tandem mass spectrometry
文献类型： 外文期刊
作者： Su, Desen 1 ; Zheng, Yunyun 1 ; Chen, Ziqiang 1 ; Chi, Yuwu 3 ;
作者机构： 1.Fujian Acad Agr Sci, Fujian Key Lab Agroprod Qual & Safety, Fuzhou 350003, Fujian, Peoples R China
2.Fujian Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Fuzhou 350003, Fujian, Peoples R China
3.Fuzhou Univ, MOE Key Lab Analyt Sci Food Safety & Biol, Fuzhou 350108, Fujian, Peoples R China
4.Fuzhou Univ, Coll Chem, Fuzhou 350108, Fujian, Peoples R China
期刊名称：ANALYST （ 影响因子：4.616； 五年影响因子：4.232 ）
ISSN： 0003-2654
年卷期： 2021 年 146 卷 5 期
页码：
收录情况： SCI
摘要： An ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method was established and validated for the simultaneous determination of six glycosidic aroma precursors in pomelo, including geraniol beta-glucoside, geraniol beta-primeveroside, linalool beta-primeveroside, benzyl beta-primeveroside, 2-phenylethyl beta-primeveroside and nerolidol beta-primeveroside. The results showed that the proposed method has the advantages of rapidity, high sensitivity, and good accuracy. Six glycosidic aroma precursors were effectively separated in a short run time (13 min), and the limit of detection, limit of quantification, recovery, and repeatability of analytes were 0.321-4.47 ng mL(-1), 1.07-14.9 ng mL(-1), 94.4-109.1%, and 5.2-14.5%, respectively. The developed method was applied to analyze the contents of glycosidic aroma precursors in different organs of pomelo plant, including leaves, flowers and fruits. The analytical result showed that glycosidic aroma precursor contents in plant leaves, flowers and fruits were in the range of 0-5964.9 mu g kg(-1), and more glycosidic aroma precursors were found in flowers than in leaves or fruits. It is envisioned that the proposed UPLC-MS/MS method have promising application in qualifying and quantifying these six glycosidic aroma precursors in pomelo.
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