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A practical and fast isolation of 12 cyclolinopeptides (linusorbs) from flaxseed oil via preparative HPLC with phenyl-hexyl column

文献类型： 外文期刊

作者： Liu, Xue 1 ; Cai, Zi-zhe 1 ; Lee, Wan Jun 1 ; Lu, Xuan-xuan 1 ; Reaney, Martin J. T. 1 ; Zhang, Jian-ping 3 ; Li, Ying 1 ;

作者机构： 1.Jinan Univ, Dept Food Sci & Engn, Guangdong Saskatchewan Oilseed Joint Lab, Guangzhou 510632, Peoples R China

2.Univ Saskatchewan, Dept Plant Sci, 51 Campus Dr, Saskatoon, SK S7N 5A8, Canada

3.Gansu Acad Agr Sci, Crop Res Inst, Lanzhou 730070, Peoples R China

关键词： Flaxseed oil; Cyclolinopeptide; Phenyl-hexyl column; Preparative HPLC

期刊名称：FOOD CHEMISTRY （ 2019影响因子：6.306； 五年影响因子：6.219 ）

ISSN： 0308-8146

年卷期： 2021 年 351 卷

页码：

收录情况： SCI

摘要： Linusorbs, known as cyclolinopeptides, are a group of cyclic hydrophobic peptides derived from flaxseed oil with various health benefits. However, the current research efforts on both the biological activities and antioxidant capacities of linusorbs are limited because of existing issues with their purification and characterization. A practical method based on preparative HPLC for isolating 12 linusorbs simultaneously was developed and factors such as the solvent selection, gradient elution program, flow rate, loaded mass, and loading concentration, were optimized. The optimum conditions were an initial acetonitrile (ACN) to water ratio of 40%, final ACN ratio of 80%, eluting time of 21 min, a flow rate of 16 mL/min, sample load of 12.5 mg, and concentration of 80 mg/mL (in methanol). The 12 linusorbs obtained were verified using off-line MS/MS, recording purities of above 95.5%. The method could serve as a practical and fast isolation method enabling further investigation of minor linusorbs.

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